Whole genome sequence of a superbug-Escherichia coli strain KAB-AI-497 isolated from the vagina of a 20 year old pregnant woman with premature rupture of membrane (PROM) in a resource limited setting, Kabale Regional Referral Hospital, in Uganda.

OBJECTIVES

The objective of the study is to sequence the whole genome of multidrug resistant E. coli strain KAB-AI-497 that causes bacterial vaginosis and implicated in premature rupture of membrane in pregnant woman.

DATA DESCRIPTION: The DNA of the E. coli strain KAB-AI-497 was extracted using the MagAttract HMW DNA Kit, and the extracted DNA was sequenced using an MGI DNBSEQ G99ARS platform. FastQC was used to perform quality control analysis and the reads were trimmed by Trimmomatic. De novo genome assembly was performed by SPAdes and it resulted to a draft assembled genome that has 5.1 Mb genome size, 153 contigs, and 50.5% GC content. Quality analysis of the assembled genome revealed it has 98.46% completeness and 0.97% contamination. The closest E. coli strain to this strain KAB-AI-497 in terms of similarity was Escherichia coli SMS-3-5 with an average nucleotide identity of 98.43% and genome coverage of 86.19%, which confirmed the species level identity of the strain. The assembled genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline which identified 4,726 protein coding genes in the strain genome. Furthermore, the annotation revealed the genome has resistant genes responsible for resistance against many antibiotic classes such as tetracycline, fluoroquinolone, and penicillin.