Frontiers in cellular and infection microbiology · 2026
Antibacterial effect of a dental adhesive containing SCH-79797 on Streptococcus mutans biofilms.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zheng Gaozhe, Zhang Lingjun, Dong Haiyun, Shao Yunhe, Deng Shuli, Sun Yan, Pan Yihuai, Wang Min
Аннотация
OBJECTIVES: Antibacterial modification of dental adhesives has emerged as a viable strategy for caries prevention. In this study, a newly identified dual-target antimicrobial, SCH-79797, known for its strong antibiofilm activity, was incorporated into a dental adhesive. Its antibiofilm performance was subsequently evaluated using a Streptococcus mutans (S. mutans) biofilm model.
METHODS: The bonding performance, polymerization behavior and surface wettability of the SCH-79797-modified adhesive were evaluated by shear bond strength testing, degree of conversion, and water contact angle measurements, respectively. Biocompatibility was examined in L929 fibroblasts using CCK-8 assays and live/dead cell staining. Antibacterial activity against biofilms was evaluated through CFU counting, MTT assays, SEM observation, and live/dead bacterial staining. In addition, biofilm virulence was evaluated by analyzing water-insoluble polysaccharide and extracellular polysaccharide (EPS) production, as well as changes in pH and lactic acid production.
RESULTS: Incorporating SCH-79797 at concentrations up to 0.5% did not adversely affect the adhesive properties and resulted in minimal cytotoxicity. Compared with the control, the SCH-79797-modified adhesive reduced bacterial viability by approximately 6 log CFU, and significantly decreased lactate production and EPS synthesis by approximately 90% and 90%, respectively.
CONCLUSIONS: These findings indicate that SCH-79797-modified dental adhesives possess promising in vitro anticaries activity against cariogenic bacteria.
METHODS: The bonding performance, polymerization behavior and surface wettability of the SCH-79797-modified adhesive were evaluated by shear bond strength testing, degree of conversion, and water contact angle measurements, respectively. Biocompatibility was examined in L929 fibroblasts using CCK-8 assays and live/dead cell staining. Antibacterial activity against biofilms was evaluated through CFU counting, MTT assays, SEM observation, and live/dead bacterial staining. In addition, biofilm virulence was evaluated by analyzing water-insoluble polysaccharide and extracellular polysaccharide (EPS) production, as well as changes in pH and lactic acid production.
RESULTS: Incorporating SCH-79797 at concentrations up to 0.5% did not adversely affect the adhesive properties and resulted in minimal cytotoxicity. Compared with the control, the SCH-79797-modified adhesive reduced bacterial viability by approximately 6 log CFU, and significantly decreased lactate production and EPS synthesis by approximately 90% and 90%, respectively.
CONCLUSIONS: These findings indicate that SCH-79797-modified dental adhesives possess promising in vitro anticaries activity against cariogenic bacteria.