Effect of some immunological markers on the level of anti-Mullerian hormone (AMH) in women infected with Toxoplasma gondii: further investigations.

OBJECTIVE

Aim: To explore the effects of T. gondii on immune levels and try to find out correlations that maybe manifest later on women who have had the infection and to assess the direct relationship between the infection and a rise in the value of anti-Mullerian hormone (AMH). Specifically, we examined the relationship between AMH and IL-1 β, IL-6, and TNF-α.

PATIENTS AND

METHODS

Materials and

Methods

Blood samples were collected from two identical groups of 80 (40 healthy control and 40 infected with T. gondii). The participants were women at an age ranging from 20 to 25 years; Group 1, healthy women who were not infected with Toxoplasma comprised the control series; Group 2, women who were Toxoplasma-infected and who visited medical facilities. Toxoplasma infection was diagnosed using the Latex Agglutination Test (LAT). Serum human interleukins (IL-1β and IL-6), tumor necrosis factor alpha (TNF-α) and anti-Mullerian hormone (AMH) were detected by Enzyme Linked Immunosorbent Assay (ELISA) method.

RESULTS

Results

Compared with the control group, infected women had significantly higher levels of human IL-1 β, IL-6, and tumor necrosis factor alpha (TNF-α). AMH concentrations correlated significantly in a positive manner with human IL-1β, IL-6, and tumor necrosis factor alpha (TNF-α).

CONCLUSION

Conclusions

Three immune parameters (IL-1 β, IL-6, and TNF-α) had elevated circulating levels, which implied the strong association between AMH and these immune variables.