Protocol for in situ transplantation of adipose stem cells into mouse epididymal white adipose tissue.

Adipose stem cells (ASCs) play a crucial role in maintaining adipose tissue homeostasis, but studying them in situ is technically challenging. Here, we present a protocol for the transplantation of ASCs into mouse epididymal white adipose tissue (eWAT). We describe steps for isolating and purifying ASCs, labeling them with a cell tracker, administering anesthesia, making an incision, and performing spot injections of ASCs into the eWAT of mice. This protocol offers broad applications for in vivo functional research of ASCs. For complete details on the use and execution of this protocol, please refer to Liao et al.1.