Journal of virological methods · 2019
Development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gI protein.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhou Xiaofeng, Wang Mingshu, Cheng Anchun, Yang Qiao, Wu Ying, Jia Renyong, Liu Mafeng, Zhu Dekang, Chen Shun, Zhang Shaqiu, Zhao Xin-Xin, Huang Juan, Mao Sai, Ou Xumin, Gao Qun, Liu Yunya, Yu Yanling, Zhang Ling, Tian Bin, Pan Leichang, Rehman Mujeeb Ur, Chen Xiaoyue
Аннотация
A colloidal gold strip (CGS) for detecting antibodies to duck plague virus (DPV) was developed. Colloidal gold-labeled DPV gI protein and goat anti-rabbit IgG were dispensed on a conjugate pad as tracers. The recombinant DPV gI protein and rabbit IgG were used as capture reagents at the test line and control line, respectively. The detection limit of this assay was 1:256. Additionally, the CGS did not react with antisera from other common duck diseases, only reacting with anti-DPV serum and yielding a specific and strong red signal. 123 serum samples were tested by CGS and enzyme-linked immunosorbent assay (ELISA), and the results showed good agreement. The CGS test results can be observed in 15 min with the naked eye, should be suitable for clinical testing and large-scale detection.