Journal of the American Society for Mass Spectrometry · 2026
Ambient Pressure Laser Desorption Ionization/Postphotoionization Mass Spectrometry Imaging.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yao Xiaobo, Wu Liutian, Wang Yaya, Lv Chunchun, Qi Keke, Xu Minggao, Liu Chengyuan, Pan Yang
Аннотация
Postphotoionization has been demonstrated to be an effective method to increase the ionization efficiency of laser desorption ionization. In order to scan samples with compact and hard characteristics from the front side, an ambient pressure laser desorption ionization in combination with a compact postphotoionization assembly (AP-LDI/PI) in reflection mode was developed. The effects of parameters, including laser energy, frequency, and stepper motor velocity, were carefully investigated. The slices of mouse cerebellum were scanned by AP-LDI/PI mass spectrometry in both positive and negative ion modes with a spatial resolution of 10 μm. In addition, the longitudinal sections of Ginkgo biloba were well imaged using AP-LDI/PI with a spatial resolution of 15 μm. The spatial distributions of metabolites, including ginkgo acids, choline, glutamate, and ethoxycoumarin, within the longitudinal sections of Ginkgo biloba were distinctly delineated.