Klinicka onkologie : casopis Ceske a Slovenske onkologicke spolecnosti · 2020
SNHG7 and FAIM2 are up-regulated and co-expressed in colorectal adenocarcinoma tissues.
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Ziaee Farinaz, Hajjari Mohammadreza, Kazeminezhad Reza Seyed, Behmanesh Mehrdad
Аннотация
BACKGROUND: The global incidence of colorectal cancer (CRC) is expected to be increased by 60% until a few years. Despite the advances in surgical and chemotherapy techniques, a significant proportion of patients with CRC have poor responses to treatments. These are the reasons that prove the importance of identifying molecular bio-markers as potential therapeutic targets. Long non-coding RNAs (lnc RNAs) participate in the initiation, development, progression, and metastasis of cancers such as CRC. Hence, this class of noncoding RNAs is known as bio-marker for cancer dia-gnosis and prognosis.
MATERIALS AND METHODS: In this experimental study, the extraction of total RNA from tissues, synthesis of complementary DNA as well as quantitative real-time polymerase chain reaction (qRT- PCR) were performed. Comparative cycle threshold method was applied to quantify the expression level of lncRNA-SNHG7 and FAIM2. The relative amount of lncRNA-SNHG7 and FAIM2 was calculated using the equation 2 -DDCT.
RESULTS: In this study, by qRT PCR, we concluded that the expression level of SNHG7, as a recently identified lncRNA and FAIM2 were increased in colorectal cancer tissues compared with normal adjacent tissues.
CONCLUSION: Our study indicates the potential importance of SNHG7 and FAIM2 expression for more studies in future.
MATERIALS AND METHODS: In this experimental study, the extraction of total RNA from tissues, synthesis of complementary DNA as well as quantitative real-time polymerase chain reaction (qRT- PCR) were performed. Comparative cycle threshold method was applied to quantify the expression level of lncRNA-SNHG7 and FAIM2. The relative amount of lncRNA-SNHG7 and FAIM2 was calculated using the equation 2 -DDCT.
RESULTS: In this study, by qRT PCR, we concluded that the expression level of SNHG7, as a recently identified lncRNA and FAIM2 were increased in colorectal cancer tissues compared with normal adjacent tissues.
CONCLUSION: Our study indicates the potential importance of SNHG7 and FAIM2 expression for more studies in future.