Journal of stomatology, oral and maxillofacial surgery · 2025
Effect of photobiomodulation on rat parotid gland cells after ionizing radiation - an experimental study in animal model - Part 1.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Durães Cristina Paixão, Fonseca Larissa Lopes, Santos Sérgio Henrique Sousa, Filho Arlen de Paulo Santiago, Silveira Marise Fagundes, Queiroz Lorena Dos Reis Pereira, Lacerda Allysson Steve Mota, de Paula Alfredo Maurício Batista, Farias Lucyana Conceição, Guimarães André Luiz Sena
Аннотация
BACKGROUND: Radiotherapy for head and neck cancers often impairs salivary gland function. Photobiomodulation (PBM) has emerged as a potential intervention to mitigate radiation-induced tissue damage. This study aimed to evaluate the effects of PBM on the parotid glands of Wistar rats subjected to ionizing radiation.
METHODS: Forty-two male Wistar rats (7-8 weeks old) were randomly assigned to seven groups (n = 6). G1: control; G2: radiotherapy only (20 Gy); G3: PBM only; G4-G7: PBM combined with radiotherapy. PBM was applied using the Laser Duo (MMOptics®) with 660 nm and/or 808 nm wavelengths, varying in dose and number of application points. The histological analysis focused on preserving the acinar area. Receiver operating characteristic (ROC) curve analysis was used to determine the threshold for preserved acinar architecture.
RESULTS: The group receiving combined PBM (660 nm + 808 nm, 54 J per gland; G5) demonstrated the highest preservation of acinar morphology, closely resembling the control. Groups receiving isolated red or infrared wavelengths (G4, G6) showed moderate protection. The lowest dose group (G7, 1.8 J) was ineffective.
CONCLUSION: PBM significantly reduced RT-induced damage to the parotid glands in a dose-dependent manner. The red laser protocol at 27 J per gland showed the highest level of tissue preservation. These findings support the potential of PBM as a protective adjunct during radiation therapy (RT) and justify further studies to standardize optimal clinical parameters.
METHODS: Forty-two male Wistar rats (7-8 weeks old) were randomly assigned to seven groups (n = 6). G1: control; G2: radiotherapy only (20 Gy); G3: PBM only; G4-G7: PBM combined with radiotherapy. PBM was applied using the Laser Duo (MMOptics®) with 660 nm and/or 808 nm wavelengths, varying in dose and number of application points. The histological analysis focused on preserving the acinar area. Receiver operating characteristic (ROC) curve analysis was used to determine the threshold for preserved acinar architecture.
RESULTS: The group receiving combined PBM (660 nm + 808 nm, 54 J per gland; G5) demonstrated the highest preservation of acinar morphology, closely resembling the control. Groups receiving isolated red or infrared wavelengths (G4, G6) showed moderate protection. The lowest dose group (G7, 1.8 J) was ineffective.
CONCLUSION: PBM significantly reduced RT-induced damage to the parotid glands in a dose-dependent manner. The red laser protocol at 27 J per gland showed the highest level of tissue preservation. These findings support the potential of PBM as a protective adjunct during radiation therapy (RT) and justify further studies to standardize optimal clinical parameters.