Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies.

The use of patient-derived endometrial epithelial organoids has revolutionized the field of uterine biology. Here, we present a protocol for generating lentivirus and transducing human-derived endometrial epithelial organoids for the study of endometrial function and pathologies. We describe steps for lentiviral production, lentiviral transduction of endometrial epithelial organoids, and endometrial epithelial organoid passaging. We then detail procedures for validating lentiviral transduction. This protocol allows for knocking down or overexpressing genes of interest, enabling investigation of specific targets in a patient-centric model.