The Journal of veterinary medical science · 2013
Cross-reactivity of secondary antibodies against African rodents and application for sero-surveillance.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Nakamura Ichiro, Hang'ombe Bernard Mudenda, Sawa Hirofumi, Kobayashi Shintaro, Orba Yasuko, Ishii Akihiro, Thomas Yuka, Isozumi Rie, Yoshimatsu Kumiko, Mweene Aaron S, Takada Ayato, Sugimoto Chihiro, Arikawa Jiro
Аннотация
A total of 466 rodents were captured in the Republic of Zambia from 2006 to 2010. Based on morphological observations and phylogenetic analyses of mitochondrial gene sequences, rodents were divided into 10 groups consisting of 39 Rattus rodents, 263 multimammate rats, 18 other Murinae rodents, 95 gerbils, 11 pouched mice, 1 giant-pouched rat, 38 fat mice and 1 dormouse. Rodent antibodies except that from Rattus were examined for their cross-reactivity to commercially available antibody detection reagents. Anti-mouse immunoglobulin G (IgG) was most cross-reactive to heterologous antibodies including multimammate rat, gerbil, pouched mouse and fat mouse. Thus, anti-mouse IgG would be a useful detection tool in serological examination of the Zambian rodent population. Preliminary sero-surveillance for plague, leptospirosis and hantavirus infection was performed by ELISA.