Science advances · 2024
Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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История · было → стало
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Crawford Ashleigh J, Forjaz André, Bons Joanna, Bhorkar Isha, Roy Triya, Schell David, Queiroga Vasco, Ren Kehan, Kramer Donald, Huang Wilson, Russo Gabriella C, Lee Meng-Horng, Wu Pei-Hsun, Shih Ie-Ming, Wang Tian-Li, Atkinson Mark A, Schilling Birgit, Kiemen Ashley L, Wirtz Denis
Аннотация
The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.