Fertility and sterility · 2012
Increased expression of macrophage colony-stimulating factor and its receptor in patients with endometriosis.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
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Budrys Nicole M, Nair Hareesh B, Liu Ya-Guang, Kirma Nameer B, Binkley Peter A, Kumar Shantha, Schenken Robert S, Tekmal Rajeshwar Rao
Аннотация
OBJECTIVE: To investigate the expression and regulation of colony-stimulating factor 1 (CSF-1) and its receptor, C-FMS, in endometriosis.
DESIGN: In vivo and vitro study.
SETTING: University-based academic medical center.
PATIENT(S): Reproductive-age women undergoing surgery for benign conditions.
INTERVENTION(S): Peritoneal and endometrial tissue samples were obtained.
MAIN OUTCOME MEASURE(S): CSF-1 and C-FMS expression.
RESULT(S): Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis.
CONCLUSION(S): Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.
DESIGN: In vivo and vitro study.
SETTING: University-based academic medical center.
PATIENT(S): Reproductive-age women undergoing surgery for benign conditions.
INTERVENTION(S): Peritoneal and endometrial tissue samples were obtained.
MAIN OUTCOME MEASURE(S): CSF-1 and C-FMS expression.
RESULT(S): Significantly higher CSF-1 levels were found in peritoneal fluid of patients with endometriosis compared with control subjects. Ectopic endometriotic tissue had 3.5-fold and 1.7-fold increases in CSF-1 and C-FMS expression, respectively, compared with eutopic tissue. Coculture of endometrial cells from either established cell lines or patient samples with peritoneal mesothelial cells (PMCs) led to increased expression of CSF-1 and C-FMS. A higher but nonsignificant increase in levels of C-FMS and CSF-1 was found in cocultures of endometrial epithelial cells from patients with endometriosis compared with those without endometriosis.
CONCLUSION(S): Increased CSF-1 levels may contribute to endometriosis lesion formation and progression. Elevation in CSF-1 after coculture of endometrial cells with PMCs suggests that endometrial tissue may be a source of peritoneal CSF-1. Increased C-FMS expression in endometrial cells from women with endometriosis cocultured with PMCs suggests that endometrial tissue involved in lesion formation is highly responsive to CSF-1 signaling.