Acta physiologica (Oxford, England) · 2025
Role of the Choroid Plexus Kir7.1 Channel in the Regulation of Mouse Cerebrospinal Fluid K+ Concentration.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Henao Juan Carlos, Burgos Johanna, Vera Erwin, Ruminot Iván, Cid L Pablo, Cornejo Isabel, Sepúlveda Francisco V
Аннотация
AIM: K+ channel Kir7.1 is prominently expressed at the apical membrane of the choroid plexus epithelium (CPE) together with the Na+-K+-ATPase pump and cotransporter NKCC1. Its unusual independence of extracellular K+ ([K+]o) suggests a key role in regulating cerebrospinal fluid (CSF) K+ concentration ([K+]CSF). We tested this hypothesis by exploring the effect of Kir7.1 inactivation or modification of its K+-dependence in mice.
METHODS: We generate conditional Kir7.1 knockout (cKO) and knockin mice carrying the M125R mutation altering Kir7.1 K+-dependence. We study the electrical properties of CPE cells in vitro as well as in vivo CSF secretion and [K+]CSF. CPE NKCC1 activity, expression and phosphorylation status were also evaluated.
RESULTS: Kir7.1 identified as the primary [K+]o-independent conductance in CPE cells, critically contributes to their membrane potential. CPE cells from Kir7.1-M125R mice exhibited a K+ conductance with direct dependence on [K+]o. While CSF secretion rates were unaltered in both cKO Kir7.1 and M125R animals, [K+]CSF was significantly decreased in cKO mice and increased in M125R mutants. Unexpectedly, NKCC1 activity was strongly inhibited in Kir7.1 cKO CPE despite unaltered expression and phosphorylation levels, but remained unaffected in M125R cells.
CONCLUSIONS: Kir7.1 imparts high K+ permeability and defines the membrane potential of CPE cells. Its unusual [K+]o-independent conductance underpins its important role in the regulation of [K+]CSF. Moreover, Kir7.1 appears crucial for NKCC1 function, likely these two proteins forming part of an apical complex with the Na+-K+-ATPase. Given the continuity of CSF with brain interstitial space, Kir7.1-mediated control of [K+]CSF might influence neuronal excitability.
METHODS: We generate conditional Kir7.1 knockout (cKO) and knockin mice carrying the M125R mutation altering Kir7.1 K+-dependence. We study the electrical properties of CPE cells in vitro as well as in vivo CSF secretion and [K+]CSF. CPE NKCC1 activity, expression and phosphorylation status were also evaluated.
RESULTS: Kir7.1 identified as the primary [K+]o-independent conductance in CPE cells, critically contributes to their membrane potential. CPE cells from Kir7.1-M125R mice exhibited a K+ conductance with direct dependence on [K+]o. While CSF secretion rates were unaltered in both cKO Kir7.1 and M125R animals, [K+]CSF was significantly decreased in cKO mice and increased in M125R mutants. Unexpectedly, NKCC1 activity was strongly inhibited in Kir7.1 cKO CPE despite unaltered expression and phosphorylation levels, but remained unaffected in M125R cells.
CONCLUSIONS: Kir7.1 imparts high K+ permeability and defines the membrane potential of CPE cells. Its unusual [K+]o-independent conductance underpins its important role in the regulation of [K+]CSF. Moreover, Kir7.1 appears crucial for NKCC1 function, likely these two proteins forming part of an apical complex with the Na+-K+-ATPase. Given the continuity of CSF with brain interstitial space, Kir7.1-mediated control of [K+]CSF might influence neuronal excitability.