The effect of bacteriostatic lubricant on group B streptococcal cultures of the female genital tract.

The purpose of this prospective investigation was to determine the effect of bacteriostatic lubricant on group B streptococcal cultures obtained from the lower genital tract of pregnant women. Fifty pregnant women with intact membranes who recently had genital tract cultures for group B streptococci were evaluated. The study group consisted of 25 women who had positive cultures, and the control group comprised 25 women who had negative cultures. All patients underwent examination with a sterile nonlubricated speculum within 7 days of the previously obtained cultures. Secretions from the endocervix and from the posterior vaginal wall near the introitus were collected with a sterile cotton-tipped applicator and inoculated directly onto a selective blood agar culture plate. The sterile speculum was removed and a digital examination performed using bacteriostatic lubricant. A second sterile speculum was then inserted into the vagina and another group B streptococcal culture was obtained. There was 100% correlation between the cultures obtained before and after the vaginal examination. We conclude that use of bacteriostatic lubricant does not alter the recovery of group B streptococci from the genital tract of pregnant women with intact membranes.