Haematologica · 2004
Primitive hematopoietic stem cells shows a polyclonal pattern in myelodysplastic syndromes.
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Guidetti Francesca, Grazioli Sandra, Capelli Francesca, Marini Claudia, Gallicchio Margherita, De Micheli Daniela, Castello Luigi, Sainaghi Pier Paolo, Fra Gian Paolo, Saglio Giuseppe, Avanzi Gian Carlo
Аннотация
BACKGROUND AND OBJECTIVES: Clonal hematopoiesis is the hallmark of myelodysplastic syndromes, but the role played by pluripotent stem cells and progenitor cells in these disorders remains unclear.
DESIGN AND METHODS: Eight female patients with myelodysplastic syndrome were studied. X-chromosome inactivation patterns were analyzed in peripheral blood granulocytes, T-lymphocytes, single colonies originating from bone marrow progenitors and pluripotent stem cells, using the human androgen receptor locus polymorphism assay.
RESULTS: Granulocytes and progenitor cells were monoclonal in 7/8 cases. Immature stem cells showed a non-clonal pattern of X-inactivation and were detectable at diagnosis in the presence of clonal hematopoiesis. T-lymphocyte clonality was heterogeneous.
INTERPRETATION AND CONCLUSIONS: In myelodysplastic syndromes, hematopoiesis may be dominated by a neoplastic clone by virtue of its biological advantage over a residual polyclonal, probably still normal, population of immature stem cells still able to grow in vitro.
DESIGN AND METHODS: Eight female patients with myelodysplastic syndrome were studied. X-chromosome inactivation patterns were analyzed in peripheral blood granulocytes, T-lymphocytes, single colonies originating from bone marrow progenitors and pluripotent stem cells, using the human androgen receptor locus polymorphism assay.
RESULTS: Granulocytes and progenitor cells were monoclonal in 7/8 cases. Immature stem cells showed a non-clonal pattern of X-inactivation and were detectable at diagnosis in the presence of clonal hematopoiesis. T-lymphocyte clonality was heterogeneous.
INTERPRETATION AND CONCLUSIONS: In myelodysplastic syndromes, hematopoiesis may be dominated by a neoplastic clone by virtue of its biological advantage over a residual polyclonal, probably still normal, population of immature stem cells still able to grow in vitro.
Предметные рубрики MeSH
AgedAged, 80 and overAnemia, RefractoryAnemia, Refractory, with Excess of BlastsBone Marrow CellsCell Culture TechniquesChromosomes, Human, XClone CellsDNA MethylationDosage Compensation, GeneticFemaleHematopoietic Stem CellsHumansLeukocytes, MononuclearMyelodysplastic SyndromesNeutrophilsPhenotypePluripotent Stem CellsStem Cells