Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2010
[The biofilm formation in Yersinia pestis strains isolated in Astrakhan region].
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Vidiaeva N A, Eroshenko G A, Kukleva L M, Shavina N Iu, Kuznetsov O S, Kutyrev V V
Аннотация
AIM: To study biofilm formation in strains of Yersinia pestis isolated in 2009 in Astrakhan region.
MATERIALS AND METHODS: Study of biofilm formation was performed on abiotic surfaces as well as on cuticle of nematode Caenorhabditis elegans. Detection of genes was performed by PCR with specific primers.
RESULTS: Study of phenotypic (fermentation of sugars and alcohols) as well as genetic (presence of plasmids, genes of chromosome region of pigmentation) characteristics of Y. pestis strains showed that they are typical for strains isolated in Astrakhan region. All isolated in 2009 strains formed well developed biofilm on abiotic surfaces and cuticle of C. elegans nematode. They contained genes of hms operon and regulatory genes hmsT and hmsP, which are necessary for formation of pigmented colonies on Congo red medium as well as biofilm on abiotic and biotic surfaces.
CONCLUSION: Strains of Y. pestis isolated in 2009 in Astrakhan region formed well developed biofilm on different types of surfaces, which could facilitate their survival in complex parasitic biocenosis of plague natural focus.
MATERIALS AND METHODS: Study of biofilm formation was performed on abiotic surfaces as well as on cuticle of nematode Caenorhabditis elegans. Detection of genes was performed by PCR with specific primers.
RESULTS: Study of phenotypic (fermentation of sugars and alcohols) as well as genetic (presence of plasmids, genes of chromosome region of pigmentation) characteristics of Y. pestis strains showed that they are typical for strains isolated in Astrakhan region. All isolated in 2009 strains formed well developed biofilm on abiotic surfaces and cuticle of C. elegans nematode. They contained genes of hms operon and regulatory genes hmsT and hmsP, which are necessary for formation of pigmented colonies on Congo red medium as well as biofilm on abiotic and biotic surfaces.
CONCLUSION: Strains of Y. pestis isolated in 2009 in Astrakhan region formed well developed biofilm on different types of surfaces, which could facilitate their survival in complex parasitic biocenosis of plague natural focus.