Muscle & nerve · 2015
Heat-Stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration.
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Oishi Yasuharu, Roy Roland R, Ogata Tomonori, Ohira Yoshinobu
Аннотация
INTRODUCTION: We investigated heat-stress effects on the adult myosin heavy chain (MyHC) profile of soleus muscle fibers at an early stage of regeneration.
METHODS: Regenerating fibers in adult rats were analyzed 2, 4, or 6 days after bupivacaine injection. Rats were heat stressed by immersion in water (42 ± 1°C) for 30 minutes 24 hours after bupivacaine injection and every other day thereafter.
RESULTS: No adult MyHC isoforms were observed after 2 days, whereas some fibers expressed only fast MyHC after 4 days. Heat stress increased fast and slow MyHC in regenerating fibers after 6 days. Regenerating fibers expressing only slow MyHC were observed only in heat-stressed muscles. Bupivacaine injection increased the number of Pax7(+) and MyoD(+) satellite cells in regenerating fibers, more so in heat-stressed rats.
CONCLUSION: The results indicate that heat stress accelerates fast-to-slow MyHC phenotype conversion in regenerating fibers via activation of satellite cells.
METHODS: Regenerating fibers in adult rats were analyzed 2, 4, or 6 days after bupivacaine injection. Rats were heat stressed by immersion in water (42 ± 1°C) for 30 minutes 24 hours after bupivacaine injection and every other day thereafter.
RESULTS: No adult MyHC isoforms were observed after 2 days, whereas some fibers expressed only fast MyHC after 4 days. Heat stress increased fast and slow MyHC in regenerating fibers after 6 days. Regenerating fibers expressing only slow MyHC were observed only in heat-stressed muscles. Bupivacaine injection increased the number of Pax7(+) and MyoD(+) satellite cells in regenerating fibers, more so in heat-stressed rats.
CONCLUSION: The results indicate that heat stress accelerates fast-to-slow MyHC phenotype conversion in regenerating fibers via activation of satellite cells.
Предметные рубрики MeSH
Anesthetics, LocalAnimalsBody WeightBupivacaineCell CountDisease Models, AnimalHSP72 Heat-Shock ProteinsHeat Stress DisordersMaleMuscle Fibers, SkeletalMuscle, SkeletalMyoD ProteinMyosin Heavy ChainsOncogene ProteinsOrgan SizePaired Box Transcription FactorsPhenotypeProtein IsoformsRatsRats, WistarRegenerationSatellite Cells, Skeletal MuscleTime Factors