[Construction of a collagen gel dermis with improved biological property].

OBJECTIVE

To improve the biological property of artificial skin.

METHODS

We have ameliorated Hansburgh and Middelkoop's method of manufacturing artificial dermis. The type I collagenase and Dispase were used to isolated neonate prepuce' dermis fibroblast. The gel dermis was constructed by compounding the fibroblast and collagen swelling solution. The property of the collagen gel dermis was measured.

RESULTS

The neonate prepuce's dermis fibroblast had property of high proliferation, high activation of the dermis, and it could secrete abundant extracellular matrix (ECM).

CONCLUSION

The collagen gel dermis is an useful dermis substitute.