The Potency of Extracellular Vesicles Derived From Mesenchymal Stem Cells Macaca fascicularis as a Candidate Cancer Therapeutic Agents.

BACKGROUND

Extracellular vesicles (EVs) are membrane vesicles secreted by cells that carry several bioactive molecules, which are currently used in biomedical research as a source of exosomes and to investigate their effect in response to cancer studies.

METHODS

EVs isolated from the supernatant of Macaca fascicularis Mesenchymal Stem Cells (MSCs), using the commercial ExoQUICK kit, and markers validation evaluated using quantitative reverse transcription Polymerase Chain Reaction (qRT-PCR). EVs were further applied on breast cancer cells (MCF-7) and colorectal cancer cells (WiDr).

RESULTS

The application of EVs to MCF-7 and WiDr cells showed growth inhibition and resulted in the cells' viability decreased compared to untreated cells. The apoptosis (BAX and BCL2) and proliferation markers (PCNA and P53) on both cells demonstrated increasing than untreated cells, expecting that the EVs prevent the cells' growth uncontrolled.

CONCLUSION

The EVs have the potential to inhibit the growth of cancer and colon cancer in vitro, determining their potential for developing as an anticancer agent.