International journal of radiation oncology, biology, physics · 2026
FLASH Proton Radiation Therapy Preserves Ocular Structure and Function Without Compromising Antitumor Efficacy.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Amit Uri, Bell Brent A, Hoyek Elias El, Velalopoulou Anastasia, Assenmacher Charles-Antoine, Kim Michele M, Verginadis Ioannis I, Radaelli Enrico, Ben-Josef Edgar, Lee Vivian, Dunaief Joshua L, Koumenis Constantinos
Аннотация
PURPOSE: To evaluate the protective effects and therapeutic efficacy of FLASH proton radiation therapy (FR) compared with standard proton radiation therapy (SR) in a preclinical model of ocular irradiation.
METHODS AND MATERIALS: Mice received bilateral ocular irradiation with SR (≤1 Gy/s) or FR (>40 Gy/s) at identical doses. Longitudinal ocular structure and function were assessed over 5 months using spectral-domain optical coherence tomography, confocal scanning laser ophthalmoscopy, electroretinography, and histopathologic analysis. Tumor control was evaluated in an intraocular B16F10 melanoma model using bioluminescence imaging and histopathology.
RESULTS: Dose-response studies identified 24 Gy as the optimal dose, producing substantial visual impairment with SR (39.5% reduction in rod a-wave amplitude, P < .001) without inducing complete blindness. SR-induced progressive ocular injury, with significant corneal edema evident by 1 month (P < .0001), advancing to ulceration by 3 months and complete corneal destruction by 5 months whereas FR maintained corneal transparency comparable to nonirradiated controls. Consistent with these findings, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis demonstrated markedly increased corneal apoptosis following SR, whereas FR showed minimal apoptotic activity. Electroretinography analysis revealed profound SR-induced functional deterioration by 5 months, with a 76% reduction in rod a-wave, 69% in rod b-wave, and 74% in cone b-wave amplitudes (all P < .0001), reflecting extensive photoreceptor and bipolar cell dysfunction. FR eyes retained near-normal responses across retinal layers. Histopathology confirmed severe SR-associated pathology, including corneal perforation, uveal inflammation, and retinal disorganization, whereas FR largely preserved ocular architecture. Importantly, FR maintained short-term antitumor efficacy equivalent to SR, with comparable reductions in tumor burden (both P < .05 vs controls, P > .05 SR vs FR).
CONCLUSIONS: FR provides substantial normal-tissue protection across multiple ocular compartments while preserving therapeutic efficacy, effectively expanding the therapeutic window for ocular radiation therapy. These findings establish proof-of-principle for clinical translation of FR to improve visual outcomes in patients with ocular and orbital malignancies without compromising tumor control.
METHODS AND MATERIALS: Mice received bilateral ocular irradiation with SR (≤1 Gy/s) or FR (>40 Gy/s) at identical doses. Longitudinal ocular structure and function were assessed over 5 months using spectral-domain optical coherence tomography, confocal scanning laser ophthalmoscopy, electroretinography, and histopathologic analysis. Tumor control was evaluated in an intraocular B16F10 melanoma model using bioluminescence imaging and histopathology.
RESULTS: Dose-response studies identified 24 Gy as the optimal dose, producing substantial visual impairment with SR (39.5% reduction in rod a-wave amplitude, P < .001) without inducing complete blindness. SR-induced progressive ocular injury, with significant corneal edema evident by 1 month (P < .0001), advancing to ulceration by 3 months and complete corneal destruction by 5 months whereas FR maintained corneal transparency comparable to nonirradiated controls. Consistent with these findings, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling analysis demonstrated markedly increased corneal apoptosis following SR, whereas FR showed minimal apoptotic activity. Electroretinography analysis revealed profound SR-induced functional deterioration by 5 months, with a 76% reduction in rod a-wave, 69% in rod b-wave, and 74% in cone b-wave amplitudes (all P < .0001), reflecting extensive photoreceptor and bipolar cell dysfunction. FR eyes retained near-normal responses across retinal layers. Histopathology confirmed severe SR-associated pathology, including corneal perforation, uveal inflammation, and retinal disorganization, whereas FR largely preserved ocular architecture. Importantly, FR maintained short-term antitumor efficacy equivalent to SR, with comparable reductions in tumor burden (both P < .05 vs controls, P > .05 SR vs FR).
CONCLUSIONS: FR provides substantial normal-tissue protection across multiple ocular compartments while preserving therapeutic efficacy, effectively expanding the therapeutic window for ocular radiation therapy. These findings establish proof-of-principle for clinical translation of FR to improve visual outcomes in patients with ocular and orbital malignancies without compromising tumor control.