Anticancer research · 2009
Demethylation of the CD133 gene is frequently detected in advanced colorectal cancer.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Hibi Kenji, Sakata Makiko, Kitamura Yo-Hei, Sakuraba Kazuma, Shirahata Atsushi, Goto Tetsuhiro, Mizukami Hiroki, Saito Mitsuo, Ishibashi Kazuyoshi, Kigawa Gaku, Nemoto Hiroshi, Sanada Yutaka
Аннотация
BACKGROUND: Recently, it has been reported that colorectal carcinoma is created and propagated by a small number of undifferentiated tumorigenic CD133(+) cells. Furthermore, it has been reported that CD133 expression is directly regulated by epigenetic modifications. Therefore, it is possible that CD133 expression by gene demethylation is related to colorectal carcinogenesis.
MATERIALS AND METHODS: The methylation status of the CD133 gene was examined in primary carcinomas and the corresponding normal tissues derived from 48 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
RESULTS: Demethylation of the CD133 gene was detected in 19 out of the 48 (40%) primary colon carcinomas, suggesting that the demethylation of CD133 is frequently observed in colorectal carcinomas. The clinicopathological data were correlated with the demethylation results. A significant difference was observed in the maximal tumor size (p=0.0222). Moreover, a trend was shown toward preferentially developing lymph node metastasis in demethylated tumors (p=0.214).
CONCLUSION: CD133 was more frequently demethylated in advanced colorectal carcinomas.
MATERIALS AND METHODS: The methylation status of the CD133 gene was examined in primary carcinomas and the corresponding normal tissues derived from 48 patients with colorectal cancer using quantitative methylation-specific PCR (qMSP) and the correlation between the methylation status and the clinicopathological findings was evaluated.
RESULTS: Demethylation of the CD133 gene was detected in 19 out of the 48 (40%) primary colon carcinomas, suggesting that the demethylation of CD133 is frequently observed in colorectal carcinomas. The clinicopathological data were correlated with the demethylation results. A significant difference was observed in the maximal tumor size (p=0.0222). Moreover, a trend was shown toward preferentially developing lymph node metastasis in demethylated tumors (p=0.214).
CONCLUSION: CD133 was more frequently demethylated in advanced colorectal carcinomas.
Предметные рубрики MeSH
AC133 AntigenAdenocarcinomaAdultAgedAged, 80 and overAntigens, CDCell DifferentiationColonColorectal NeoplasmsDNA MethylationFemaleGene Expression Regulation, NeoplasticGlycoproteinsHumansLymphatic MetastasisMaleMiddle AgedNeoplasm StagingPeptidesPolymerase Chain ReactionPrognosisPromoter Regions, GeneticRectumYoung Adult