Communications biology · 2022
Oestrogen-dependent hypothalamic oxytocin expression with changes in feeding and body weight in female rats.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Nishimura Kazuaki, Yoshino Kiyoshi, Ikeda Naofumi, Baba Kazuhiko, Sanada Kenya, Akiyama Yasuki, Nishimura Haruki, Tanaka Kentaro, Sonoda Satomi, Ueno Hiromichi, Yoshimura Mitsuhiro, Maruyama Takashi, Hachisuga Toru, Ueta Yoichi
Аннотация
Oxytocin (OXT) is produced in the hypothalamic nuclei and secreted into systemic circulation from the posterior pituitary gland. In the central nervous system, OXT regulates behaviours including maternal and feeding behaviours. Our aim is to evaluate whether oestrogen regulates hypothalamic OXT dynamics. Herein, we provide the first evidence that OXT dynamics in the hypothalamus vary with sex and that oestrogen may modulate dynamic changes in OXT levels, using OXT-mRFP1 transgenic rats. The fluorescence intensity of OXT-mRFP1 and expression of the OXT and mRFP1 genes in the hypothalamic nuclei is highest during the oestrus stage in female rats and decreased significantly in ovariectomised rats. Oestrogen replacement caused significant increases in fluorescence intensity and gene expression in a dose-related manner. This is also demonstrated in the rats' feeding behaviour and hypothalamic Fos neurons using cholecystokinin-8 and immunohistochemistry. Hypothalamic OXT expression is oestrogen-dependent and can be enhanced centrally by the administration of oestrogen.