The European journal of surgery = Acta chirurgica · 1999
Intraperitoneal transplantation of isologous mesothelial cells for prevention of adhesions.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Bertram P, Tietze L, Hoopmann M, Treutner K H, Mittermayer C, Schumpelick V
Аннотация
OBJECTIVE: To study the effect of transplantation of mesothelial cells on the formation of adhesions after peritoneal abrasion.
DESIGN: Animal study.
SETTING: Teaching hospital, Germany.
ANIMALS: 30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group.
INTERVENTIONS: The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed.
MAIN OUTCOME MEASURES: Measurements of the areas of adhesions by computer aided morphometry.
RESULTS: The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01).
CONCLUSION: Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.
DESIGN: Animal study.
SETTING: Teaching hospital, Germany.
ANIMALS: 30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group.
INTERVENTIONS: The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed.
MAIN OUTCOME MEASURES: Measurements of the areas of adhesions by computer aided morphometry.
RESULTS: The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01).
CONCLUSION: Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.