Journal of thrombosis and haemostasis : JTH · 2024
Metalloproteomic analysis of hemophilic arthropathy synovial tissue: insights into metal overload and pathogenesis.
СтатьяСравнительное исследованиеНазвание и аннотация: Перевод в очереди
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Luo Dasheng, Zheng Liujie, Ding Mingyang, Yu Defu, Chen Tao, Zheng Ke, Jing Juehua, Yao Yunfeng
Аннотация
BACKGROUND: Hemophilic arthropathy (HA) is a joint disease characterized by local iron overload stemming from erythrocyte rupture and closely linked to synovial lesions. However, the precise molecular characteristics of clinical HA synovial samples remain to be defined.
OBJECTIVES: To gain insight into HA synovial tissue lesions, we utilized a metalloprotein strategy to compare the metal and protein spectra of HA with those of osteoarthritis and rheumatoid arthritis.
METHODS: We collected synovial samples from patients with HA, osteoarthritis, and rheumatoid arthritis. Tissue metal and protein profiles were obtained by metallomics and proteomics, respectively. Finally, metalloproteomics strategies compared metal content, proteins, metalloproteins, and the life processes involved.
RESULTS: Our metallomics analysis revealed an explicit increase in heavy metal content, particularly arsenic and mercury, in HA synovial samples. Through proteomics, we delineated specific metalloproteins and identified correlations between metals and pathways.
CONCLUSION: These findings yield valuable insights into the pathogenesis of HA and offer potential therapeutic targets for conditions characterized by iron overload.
OBJECTIVES: To gain insight into HA synovial tissue lesions, we utilized a metalloprotein strategy to compare the metal and protein spectra of HA with those of osteoarthritis and rheumatoid arthritis.
METHODS: We collected synovial samples from patients with HA, osteoarthritis, and rheumatoid arthritis. Tissue metal and protein profiles were obtained by metallomics and proteomics, respectively. Finally, metalloproteomics strategies compared metal content, proteins, metalloproteins, and the life processes involved.
RESULTS: Our metallomics analysis revealed an explicit increase in heavy metal content, particularly arsenic and mercury, in HA synovial samples. Through proteomics, we delineated specific metalloproteins and identified correlations between metals and pathways.
CONCLUSION: These findings yield valuable insights into the pathogenesis of HA and offer potential therapeutic targets for conditions characterized by iron overload.