Haemophilia : the official journal of the World Federation of Hemophilia · 2020
Proteoglycan synthesis rate as a novel method to measure blood-induced cartilage degeneration in non-haemophilic and haemophilic rats.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Pulles Astrid E, Vøls Kåre K, Christensen Kristine R, Coeleveld Katja, Hansen Axel K, van Vulpen Lize F D, Petersen Maj, Mastbergen Simon C, Roepstorff Kirstine, Schutgens Roger E G, Kjelgaard-Hansen Mads, Lafeber Floris P J G
Аннотация
INTRODUCTION: Haemophilic animal models are used to study blood-induced cartilage damage, but quantitative and sensitive outcome measures are needed.
AIM: To develop a novel quantitative method for detecting early cartilage degeneration in a haemophilic rat model of blood-induced joint damage.
METHODS: The 35 Sulphate incorporation (35 SO4 2- assay) was applied to tibial and patellar cartilage of wild-type rats to quantify baseline proteoglycan synthesis and to evaluate the effect of 4-day blood exposure in vitro. Next, haemarthrosis was induced in 39 FVIII-deficient rats and characterized by changes in knee joint diameter and development of bone pathology (using micro-CT). Four- and 16-day posthaemarthrosis proteoglycan synthesis rate (PSR) was assessed using the 35 SO4 2- assay, with the contralateral knee as control.
RESULTS: In vitro, a decrease in PSR in tibial and patellar cartilage was demonstrated following blood exposure. In vivo, joint diameter and development of bone pathology confirmed successful induction of haemarthrosis. In the blood-exposed knee, tibial and patellar PSR was inhibited 4 and 16 days after induced haemarthrosis. Interestingly, at day 16 the proteoglycan synthesis in the contralateral knee was also inhibited to an extent correlating with that of the blood-exposed knee.
CONCLUSION: For the first time, early changes in cartilage matrix synthesis upon blood exposure were quantified with the 35 SO4 2- assay in a haemophilic rat model, establishing this assay as a novel method to study blood-induced cartilage damage.
AIM: To develop a novel quantitative method for detecting early cartilage degeneration in a haemophilic rat model of blood-induced joint damage.
METHODS: The 35 Sulphate incorporation (35 SO4 2- assay) was applied to tibial and patellar cartilage of wild-type rats to quantify baseline proteoglycan synthesis and to evaluate the effect of 4-day blood exposure in vitro. Next, haemarthrosis was induced in 39 FVIII-deficient rats and characterized by changes in knee joint diameter and development of bone pathology (using micro-CT). Four- and 16-day posthaemarthrosis proteoglycan synthesis rate (PSR) was assessed using the 35 SO4 2- assay, with the contralateral knee as control.
RESULTS: In vitro, a decrease in PSR in tibial and patellar cartilage was demonstrated following blood exposure. In vivo, joint diameter and development of bone pathology confirmed successful induction of haemarthrosis. In the blood-exposed knee, tibial and patellar PSR was inhibited 4 and 16 days after induced haemarthrosis. Interestingly, at day 16 the proteoglycan synthesis in the contralateral knee was also inhibited to an extent correlating with that of the blood-exposed knee.
CONCLUSION: For the first time, early changes in cartilage matrix synthesis upon blood exposure were quantified with the 35 SO4 2- assay in a haemophilic rat model, establishing this assay as a novel method to study blood-induced cartilage damage.