Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.] · 2009
Detection of bacteria by fluorescence in situ hybridization in culture-negative soft tissue filler lesions.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Bjarnsholt Thomas, Tolker-Nielsen Tim, Givskov Michael, Janssen Martin, Christensen Lise Hanne
Аннотация
BACKGROUND: Adverse reactions to polyacrylamide gel occur as swellings or nodules, and controversy exists whether these are due to bacterial infection or an autoimmune reaction to the filler.
OBJECTIVES: Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization.
RESULTS: Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates.
CONCLUSION: This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment.
OBJECTIVES: Biopsies from culture-negative long-lasting nodules after injection with different types of polyacrylamide gel were examined with a combination of Gram stain and fluorescence in situ hybridization.
RESULTS: Bacteria were detected in biopsies from seven of eight patients. They inhabited gel and intervening tissue and tended to lie in aggregates.
CONCLUSION: This study supports the assumption that infection with bacteria in aggregates causes culture-negative late adverse reactions to polyacrylamide gel, suggesting a biofilm environment.