Воздействие долгосрочного замороженного хранения на стабильность индекса свободной цепи освещения каппы.
Обработка статьи · этапы, ошибки и управление
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Schmidauer Martin, Berek Klaus, Bsteh Gabriel, Auer Michael, Barket Robert, Di Pauli Franziska, Hassler Michaela, Milosajevic Dejan, Zinganell Anne, Walde Janette, Deisenhammer Florian, Hegen Harald
Аннотация
Оригинал публикации
Effect of long-term frozen storage on stability of kappa free light chain index.
OBJECTIVES: To investigate whether frozen storage duration influences κ-FLC index.
METHODS: CSF and serum samples of patients with multiple sclerosis collected for routine diagnostic purposes had been stored at -20 °C. κ-FLC and albumin concentrations were measured at two different timepoints, i.e. before and after storage. The κ-FLC index was calculated as (CSF κ-FLC/serum κ-FLC)/(CSF albumin/serum albumin).
RESULTS: A total of 70 patients were included showing median CSF κ-FLC concentration of 0.25 (25th-75th percentile: 0.12-0.43) mg/dL, serum κ-FLC concentration of 1.05 (0.86-1.34) mg/dL, CSF albumin concentration of 17.7 (14.6-26.1) mg/dL and serum albumin concentration of 4230 (3898-4488) mg/dL. The κ-FLC index was 44 (25-108). With increasing frozen storage duration, the absolute concentrations of CSF κ-FLC, serum κ-FLC, CSF albumin and serum albumin decreased, while the κ-FLC index remained stable. The observed changes in absolute concentrations evened out by using CSF/serum ratios of κ-FLC and albumin when calculating the κ-FLC index.
CONCLUSIONS: Frozen storage at -20 °C has no relevant impact on κ-FLC index.