Frontiers in neuroscience · 2026
High resolution, contrast-enhanced X-ray microscopy of the ex vivo human cochlea: technical feasibility and biometric case analysis.
СтатьяНазвание и аннотация: Перевод в очереди
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Jang Sophie S, Malhotra Charvi, McInturff Stephen, Batts Shelley, Stankovic Konstantina M
Аннотация
BACKGROUND: The human cochlea is a fragile, spiral-shaped sensory organ for hearing that is deeply embedded within the dense otic capsule bone, making accurate post-mortem anatomical studies difficult when using conventional histological approaches requiring sectioning. X-ray microscopy (XRM) is a non-destructive radiological imaging modality that combines geometric and optical magnification to achieve high-resolution 3D visualization.
METHODS: Here, we evaluated the ability of XRM to resolve the entire inner ear, including delineating soft tissue and bony structures, in trimmed human temporal bones ex vivo following lumen perfusion with a reversible iodine-based contrast-enhancing solution. After optimizing contrast incubation conditions, XRM datasets were acquired, reconstructed, and used to generate 3D volume renderings of the inner ear.
RESULTS: XRM enabled visualization of the entire cochlea and vestibular system, from millimeter-scale labyrinthine anatomy to submillimeter and micrometer-scale cochlear structures, including spiral ganglion nerve fiber bundles and sensory cell rows. Quantitative analysis in a representative case demonstrated an increasing width-to-height ratio of the organ of Corti from the basal to the apical cochlear turns, consistent with histology images and prior anatomical studies.
CONCLUSION: These findings demonstrate that XRM provides high-resolution, sectioning-free ex vivo 3D visualization of human cochlear anatomy and may enable quicker and more faithful correlation of cochlear pathology with otologic disorders than traditional histology.
METHODS: Here, we evaluated the ability of XRM to resolve the entire inner ear, including delineating soft tissue and bony structures, in trimmed human temporal bones ex vivo following lumen perfusion with a reversible iodine-based contrast-enhancing solution. After optimizing contrast incubation conditions, XRM datasets were acquired, reconstructed, and used to generate 3D volume renderings of the inner ear.
RESULTS: XRM enabled visualization of the entire cochlea and vestibular system, from millimeter-scale labyrinthine anatomy to submillimeter and micrometer-scale cochlear structures, including spiral ganglion nerve fiber bundles and sensory cell rows. Quantitative analysis in a representative case demonstrated an increasing width-to-height ratio of the organ of Corti from the basal to the apical cochlear turns, consistent with histology images and prior anatomical studies.
CONCLUSION: These findings demonstrate that XRM provides high-resolution, sectioning-free ex vivo 3D visualization of human cochlear anatomy and may enable quicker and more faithful correlation of cochlear pathology with otologic disorders than traditional histology.