Zhen ci yan jiu = Acupuncture research · 2026
[Mechanisms of electroacupuncture at Foot Shaoyang meridian acupoints on inflammatory response in postmenopausal osteoporosis rats based on the PI3K/AKT signaling pathway].
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Lü Xing-Min, Tang Cai, Rong Yi, Zou Ling-Ling, Xu Mao-Ting, Zhang Lu, Chen Gui-Quan
Аннотация
OBJECTIVES: To investigate the effect of electroacupuncture (EA) at Foot Shaoyang meridian acupoints on the inflammatory response in postmenopausal osteoporosis rats by regulating the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.
METHODS: Forty Sprague-Dawley (SD) rats were randomly divided into a sham operation group (n=10), and the remaining rats underwent bilateral ovariectomy to establish a postmenopausal osteoporosis model after 3 months. The model rats were further randomized into a model group, an EA group, and an EA + inhibitor group (n=10). Rats of The EA group received EA at bilateral "Huantiao" (GB30), "Yanglingquan" (GB34) (dense-sparse wave, 2 Hz/15 Hz, 0.2-0.6 mA), and acupuncture at "Xuanzhong" (GB39), 10 min each time, 5 times a week for 8 weeks, with alternate left-right acupoints. Rats of the EA + inhibitor group received intraperitoneal injection of the PI3K inhibitor LY294002 (10 mg/kg) twice a week for 8 weeks based on the EA treatment. Micro-CT was used to detect the microstructural and morphometric indices of the femoral bone tissue, including bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), and trabecular separation (Tb.Sp). HE staining was performed to observe the pathological morphology of the femoral tissue, and tartrate-resistant acid phosphatase (TRAP) staining was used to observe the number of osteoclasts. Immunohistochemical staining was employed to detect the expressions of osteogenic markers Runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and type I collagen (Collagen I). The contents of serum interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), as well as their mRNA expressions in bone tissue, were detected by ELISA and real-time fluorescent quantitative PCR, respectively. Western blot was used to detect the protein expressions of the PI3K/AKT pathway in femoral tissue.
RESULTS: In the model group, bone trabeculae were significantly thinned, sparsely and disorderly arranged, the bone marrow cavity was expanded, and the number of osteoclasts was significantly increased;the EA group showed improved bone trabecular structure and reduced osteoclast number. Compared with the sham operation group, the model group had significantly lower BMD, BV/TV, Tb.N, positive expressions of Runx2, OCN, and Collagen I in bone tissue, and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression (P<0.01), while Tb.Sp, serum IL-6, IL-1β, TNF-α contents and their mRNA expressions in bone tissue were significantly higher (P<0.01). Compared with the model group, the EA group had significantly higher BMD, BV/TV, Tb.N, positive expressions of Runx2, OCN, and Collagen I in bone tissue, and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression (P<0.01, P<0.05), while Tb.Sp, serum IL-6, IL-1β, TNF-α contents and their mRNA expressions in bone tissue were significantly lower (P<0.01). Compared with the EA group, all the above indices except Tb.Sp were reversed in the EA + inhibitor group (P<0.01, P<0.05).
CONCLUSIONS: EA at Foot Shaoyang meridian acupoints can alleviate inflammatory responses and bone loss in postmenopausal osteoporosis rats, and its mechanism may be related to the activation of the PI3K/AKT pathway.
METHODS: Forty Sprague-Dawley (SD) rats were randomly divided into a sham operation group (n=10), and the remaining rats underwent bilateral ovariectomy to establish a postmenopausal osteoporosis model after 3 months. The model rats were further randomized into a model group, an EA group, and an EA + inhibitor group (n=10). Rats of The EA group received EA at bilateral "Huantiao" (GB30), "Yanglingquan" (GB34) (dense-sparse wave, 2 Hz/15 Hz, 0.2-0.6 mA), and acupuncture at "Xuanzhong" (GB39), 10 min each time, 5 times a week for 8 weeks, with alternate left-right acupoints. Rats of the EA + inhibitor group received intraperitoneal injection of the PI3K inhibitor LY294002 (10 mg/kg) twice a week for 8 weeks based on the EA treatment. Micro-CT was used to detect the microstructural and morphometric indices of the femoral bone tissue, including bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), and trabecular separation (Tb.Sp). HE staining was performed to observe the pathological morphology of the femoral tissue, and tartrate-resistant acid phosphatase (TRAP) staining was used to observe the number of osteoclasts. Immunohistochemical staining was employed to detect the expressions of osteogenic markers Runt-related transcription factor 2 (Runx2), osteocalcin (OCN), and type I collagen (Collagen I). The contents of serum interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), as well as their mRNA expressions in bone tissue, were detected by ELISA and real-time fluorescent quantitative PCR, respectively. Western blot was used to detect the protein expressions of the PI3K/AKT pathway in femoral tissue.
RESULTS: In the model group, bone trabeculae were significantly thinned, sparsely and disorderly arranged, the bone marrow cavity was expanded, and the number of osteoclasts was significantly increased;the EA group showed improved bone trabecular structure and reduced osteoclast number. Compared with the sham operation group, the model group had significantly lower BMD, BV/TV, Tb.N, positive expressions of Runx2, OCN, and Collagen I in bone tissue, and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression (P<0.01), while Tb.Sp, serum IL-6, IL-1β, TNF-α contents and their mRNA expressions in bone tissue were significantly higher (P<0.01). Compared with the model group, the EA group had significantly higher BMD, BV/TV, Tb.N, positive expressions of Runx2, OCN, and Collagen I in bone tissue, and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression (P<0.01, P<0.05), while Tb.Sp, serum IL-6, IL-1β, TNF-α contents and their mRNA expressions in bone tissue were significantly lower (P<0.01). Compared with the EA group, all the above indices except Tb.Sp were reversed in the EA + inhibitor group (P<0.01, P<0.05).
CONCLUSIONS: EA at Foot Shaoyang meridian acupoints can alleviate inflammatory responses and bone loss in postmenopausal osteoporosis rats, and its mechanism may be related to the activation of the PI3K/AKT pathway.