Zhongguo zhen jiu = Chinese acupuncture & moxibustion · 2025
[Effect of moxibustion at "Zusanli" (ST36) on amino acid metabolism of knee cartilage in KOA rats].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Liu Lei, Wei Xia, Gao Zizhan, Duan Wenxiu, Yu Qing, Wang Jie, Cai Ronglin, Wu Zijian
Аннотация
OBJECTIVE: To observe the effect of moxibustion at "Zusanli" (ST36) on amino acid metabolism of knee cartilage in the rats with knee osteoarthritis (KOA), and explore the mechanism of moxibustion for alleviating KOA cartilage injury.
METHODS: Thirty male SD rats were randomly divided into a normal group, a model group, and a moxibustion group, 10 rats in each one. The KOA models were prepared by injecting the mixed solution of L-cysteine and papain into the bilateral knee joint cavities of rats in the model group and the moxibustion group. In the moxibustion group, moxibustion was applied to bilateral "Zusanli" (ST36), 30 min each time, once daily and for consecutive 30 days. Before and after modeling, as well as after intervention, the knee joint diameter was measured. After intervention, the mechanical pain threshold was detected, the morphology of the cartilage tissue in the knee joint was observed using HE staining, and the ultrastructure of chondrocytes was observed under transmission electron microscopy; the levels of matrix metalloproteinase-13 (MMP-13) and interleukin-1β (IL-1β) in synovial tissue were detected using ELISA and the expression of cartilage amino acid metabolites was detected using liquid chromatography-mass spectrometry (LC-MS).
RESULTS: Compared with the normal group, in the model group, the cartilage surface of the knee joint was rough and dark, the cells appeared clustering, nuclear pyknosis and vacuolar degeneration, the endoplasmic reticulum was obviously reduced and abnormal in structure; the diameter of the knee joint increased (P<0.01), the mechanical pain threshold decreased (P<0.05), the contents of MMP-13 and IL-1β in the knee joint synovium were elevated (P<0.01), and the expression levels of proline, glycine, lysine, aspartate, alanine, methionine, and serine in the knee joint cartilage rose (P<0.05). When compared with the model group, in the moxibustion group, the injury of knee cartilage tissue was alleviated, the diameter of knee joint was reduced (P<0.01), and the mechanical pain threshold was elevated (P<0.05); the contents of MMP-13 and IL-1β in knee joint synovium decreased (P<0.05), and the expression levels of proline, glycine, lysine, aspartate, histidine, glutamate in knee joint cartilage decreased (P<0.05).
CONCLUSION: Moxibustion at "Zusanli" (ST36) alleviates joint swelling, pain, chondrocyte injury, and synovitis in KOA model rats; and exerts its anti-inflammatory, analgesic, and cartilage protective effect by modulating immune response and synthesis and degradation of cartilage in KOA through metabolic pathways of amino acids such as glycine, proline, aspartate and lysine.
METHODS: Thirty male SD rats were randomly divided into a normal group, a model group, and a moxibustion group, 10 rats in each one. The KOA models were prepared by injecting the mixed solution of L-cysteine and papain into the bilateral knee joint cavities of rats in the model group and the moxibustion group. In the moxibustion group, moxibustion was applied to bilateral "Zusanli" (ST36), 30 min each time, once daily and for consecutive 30 days. Before and after modeling, as well as after intervention, the knee joint diameter was measured. After intervention, the mechanical pain threshold was detected, the morphology of the cartilage tissue in the knee joint was observed using HE staining, and the ultrastructure of chondrocytes was observed under transmission electron microscopy; the levels of matrix metalloproteinase-13 (MMP-13) and interleukin-1β (IL-1β) in synovial tissue were detected using ELISA and the expression of cartilage amino acid metabolites was detected using liquid chromatography-mass spectrometry (LC-MS).
RESULTS: Compared with the normal group, in the model group, the cartilage surface of the knee joint was rough and dark, the cells appeared clustering, nuclear pyknosis and vacuolar degeneration, the endoplasmic reticulum was obviously reduced and abnormal in structure; the diameter of the knee joint increased (P<0.01), the mechanical pain threshold decreased (P<0.05), the contents of MMP-13 and IL-1β in the knee joint synovium were elevated (P<0.01), and the expression levels of proline, glycine, lysine, aspartate, alanine, methionine, and serine in the knee joint cartilage rose (P<0.05). When compared with the model group, in the moxibustion group, the injury of knee cartilage tissue was alleviated, the diameter of knee joint was reduced (P<0.01), and the mechanical pain threshold was elevated (P<0.05); the contents of MMP-13 and IL-1β in knee joint synovium decreased (P<0.05), and the expression levels of proline, glycine, lysine, aspartate, histidine, glutamate in knee joint cartilage decreased (P<0.05).
CONCLUSION: Moxibustion at "Zusanli" (ST36) alleviates joint swelling, pain, chondrocyte injury, and synovitis in KOA model rats; and exerts its anti-inflammatory, analgesic, and cartilage protective effect by modulating immune response and synthesis and degradation of cartilage in KOA through metabolic pathways of amino acids such as glycine, proline, aspartate and lysine.