Stomatologiia · 2025
[Use of simvastatin and pentoxifylline to potentiate the action of BMP-2 in modelled postmenopausal osteoporosis].
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Kuznetsova V S, Sinelnikova V A, Rudik I S, Vasilyev A V
Аннотация
THE AIM OF THE STUDY: To study in vivo the biological properties of simvastatin and pentoxifylline and their ability to potentiate the action of bone morphogenetic protein-2 (BMP-2) for bone tissue regeneration in conditions of simulated postmenopausal osteoporosis.
MATERIALS AND METHODS: The study was performed on 60 female Wistar rats. To create conditions that provoke the development of osteoporosis, 30 rats underwent ovariectomy; 30 animals underwent false ovariectomy without removing the ovaries. After 8 weeks, changes in the levels of osteoporosis markers, osteocalcin (OCN) and type I collagen C-telopeptide (CTX-1), in blood serum were assessed in all animals by enzyme immunoassay. To assess the osteoinductive properties, all animals underwent subcutaneous implantation of drugs and their combinations with BMP-2. In each group, both with ovariectomy and in falsely operated animals, 5 subgroups of 6 animals each were formed: simvastatin (0.13 mg/ml) was implanted in group 1, pentoxifylline (0.14 mg/ml) in group 2, BMP-2 (100 mcg/ml) in group 3, simvastatin and BMP-2 (0.13 mg/ml and 100 mg/ml) in group 4, pentoxifylline and BMP-2 (0.14 mg/ml and 100 mcg/ml) in group 5.
RESULTS: Simvastatin and pentoxifylline did not have osteoinductive properties. During BMP-2 implantation, the volume of newly formed bone tissue was 0.5±0.2% in the group of rats with ovariectomy and 2.8± 1.5% in falsely operated animals. In turn, implantation of simvastatin with BMP-2 caused the formation of bone tissue in the volume of 7.4±4.2% and 5.4±1.5%, and pentoxifylline - 0.01±0.001% and 2.1±1.5% in the groups of rats with ovariectomy and falsely operated animals, respectively.
CONCLUSION: The results obtained demonstrate the possibilities of using well-known pharmacological substances to enhance the osteoinductive effect of BMP-2 and can be used to create new effective bone graft materials in dentistry and maxillofacial surgery.
MATERIALS AND METHODS: The study was performed on 60 female Wistar rats. To create conditions that provoke the development of osteoporosis, 30 rats underwent ovariectomy; 30 animals underwent false ovariectomy without removing the ovaries. After 8 weeks, changes in the levels of osteoporosis markers, osteocalcin (OCN) and type I collagen C-telopeptide (CTX-1), in blood serum were assessed in all animals by enzyme immunoassay. To assess the osteoinductive properties, all animals underwent subcutaneous implantation of drugs and their combinations with BMP-2. In each group, both with ovariectomy and in falsely operated animals, 5 subgroups of 6 animals each were formed: simvastatin (0.13 mg/ml) was implanted in group 1, pentoxifylline (0.14 mg/ml) in group 2, BMP-2 (100 mcg/ml) in group 3, simvastatin and BMP-2 (0.13 mg/ml and 100 mg/ml) in group 4, pentoxifylline and BMP-2 (0.14 mg/ml and 100 mcg/ml) in group 5.
RESULTS: Simvastatin and pentoxifylline did not have osteoinductive properties. During BMP-2 implantation, the volume of newly formed bone tissue was 0.5±0.2% in the group of rats with ovariectomy and 2.8± 1.5% in falsely operated animals. In turn, implantation of simvastatin with BMP-2 caused the formation of bone tissue in the volume of 7.4±4.2% and 5.4±1.5%, and pentoxifylline - 0.01±0.001% and 2.1±1.5% in the groups of rats with ovariectomy and falsely operated animals, respectively.
CONCLUSION: The results obtained demonstrate the possibilities of using well-known pharmacological substances to enhance the osteoinductive effect of BMP-2 and can be used to create new effective bone graft materials in dentistry and maxillofacial surgery.