The Journal of allergy and clinical immunology · 2025
IgE-activated human lung mast cells preferentially release prostaglandin D2, thromboxane A2, and cysteinyl-leukotrienes.
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Johnsson Anna-Karin, Kolmert Johan, Rönnberg Elin, Säfholm Jesper, Al-Ameri Mamdoh, Sachs Erik, Vali Kasra, Wheelock Craig E, Dahlén Sven-Erik, Nilsson Gunnar P
Аннотация
BACKGROUND: The capacity of human lung mast cells (HLMC) to biosynthesize lipid mediators other than prostaglandin D2 (PGD2) and the cysteinyl leukotrienes is not established. We therefore profiled lipid mediator metabolites in IgE-activated HLMC.
METHODS: A liquid chromatography-tandem mass spectrometry platform including 107 metabolites of main endogenous polyunsaturated fatty acids was applied to supernatants of HLMC and for comparison on material from in vitro-developed cord blood mast cells. Involved pathways were defined by the use of specific pharmacologic inhibitors and transcriptomic analysis of the expression of relevant enzymes.
RESULTS: IgE activation of HLMC profoundly increased the release of leukotriene C4 (>500 times), PGD2 (>200 times), and thromboxane B2 (>100 times). In contrast, only minimal production of prostaglandin E2, leukotriene B4, or lipoxin A4 was detected. The majority of the quantified compounds (n = 50) were metabolites of arachidonic acid, whereas levels of metabolites from other polyunsaturated fatty acids were low. Despite the COX-2 transcript being the most abundant species, all prostanoids, as well as lipoxin A4 and 15-HETE, were solely biosynthesized in reactions catalyzed by COX-1. There was no apparent shunting between the main enzymatic pathways when COX-1 or 5-LOX were inhibited. Similar results were obtained in the cord blood mast cell model.
CONCLUSIONS: There is a high degree of specialization in HLMC with a strong and specific increase in cysteinyl leukotrienes and PGD2 but also the bronchoconstrictor thromboxane A2 after IgE-dependent activation.
METHODS: A liquid chromatography-tandem mass spectrometry platform including 107 metabolites of main endogenous polyunsaturated fatty acids was applied to supernatants of HLMC and for comparison on material from in vitro-developed cord blood mast cells. Involved pathways were defined by the use of specific pharmacologic inhibitors and transcriptomic analysis of the expression of relevant enzymes.
RESULTS: IgE activation of HLMC profoundly increased the release of leukotriene C4 (>500 times), PGD2 (>200 times), and thromboxane B2 (>100 times). In contrast, only minimal production of prostaglandin E2, leukotriene B4, or lipoxin A4 was detected. The majority of the quantified compounds (n = 50) were metabolites of arachidonic acid, whereas levels of metabolites from other polyunsaturated fatty acids were low. Despite the COX-2 transcript being the most abundant species, all prostanoids, as well as lipoxin A4 and 15-HETE, were solely biosynthesized in reactions catalyzed by COX-1. There was no apparent shunting between the main enzymatic pathways when COX-1 or 5-LOX were inhibited. Similar results were obtained in the cord blood mast cell model.
CONCLUSIONS: There is a high degree of specialization in HLMC with a strong and specific increase in cysteinyl leukotrienes and PGD2 but also the bronchoconstrictor thromboxane A2 after IgE-dependent activation.