A comprehensive portable electrochemical sensing system solution: a case study on malachite green detection.

Developing portable electrochemical sensing platforms for on-site analysis remains a critical challenge for food safety and environmental monitoring due to the high cost and immobility of commercial instrumentation. In this study, we engineered a functionally integrated, miniaturized electrochemical circuit board that seamlessly supports both two- and three-electrode measurement systems. Validated through system simulations and experimental testing, the analytical capability of this custom platform successfully meets the requirements of portable electrochemical detection. As a proof-of-concept for food safety screening, we fabricated mesoporous silica-modified electrodes and integrated them with our portable system to quantitatively detect malachite green (MG) in fish sample extracts, displaying a response trend aligned with commercial systems. In addition, the successful implementation of alternative electrochemical methods further highlights the platform's exceptional potential for the point-of-care testing (POCT) of diverse environmental and biological targets.