Technical benchmarking of library preparation kits for accurate WGS of picogram-level Mycobacterium tuberculosis DNA without whole-genome amplification.

Whole‑genome sequencing of GC rich Mycobacterium tuberculosis is constrained by low DNA input. We compared two standard and two specialised low‑input library preparation kits using picogram‑level DNA without whole‑genome amplification. Standard enzymatic fragmentation kits produced more consistent coverage and variant fidelity across inputs.