Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2009
Screening of candidated molecular markers in polymyositis using GenMAPP software.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zhu Wu, Li Jing
Аннотация
OBJECTIVE: To screen and identify candidated molecular markers in polymyositis (PM).
METHODS: The differential gene expression profile in PM was constructed. Gene MicroArray Pathway Profiler (GenMAPP) software was used to analyze the changes of microassay gene expression profiles and to identify candidate molecular markers in PM. The results of differential gene expression profile were furthermore verified by real-time quantitative RT-PCR,and Western blot were performed to confirm the differential expressed gene.
RESULTS: Compared with normal muscle tissues, we found 119 genes, including 8 expressed sequence tag (EST) genes with 3 folds differential gene expression among 17 000 genes in PM. A total of 64 genes and 47 genes were found up-regulated and down-regulated in PM, respectively. These genes were found to be associated with cell proliferation, signal transduction, cell cycle, gene transcription, and DNA damage and repair. RT-PCR and Western blot showed that the expression levels of NF-kappaB, TNF-alpha, and IL-1alpha genes were remarkably up-regulated in PM.
CONCLUSION: The development of PM might be regulated by multi-gene expression and NF-kappaB, TNF-alpha, and IL-1alpha genes might be candidate molecular markers of PM.
METHODS: The differential gene expression profile in PM was constructed. Gene MicroArray Pathway Profiler (GenMAPP) software was used to analyze the changes of microassay gene expression profiles and to identify candidate molecular markers in PM. The results of differential gene expression profile were furthermore verified by real-time quantitative RT-PCR,and Western blot were performed to confirm the differential expressed gene.
RESULTS: Compared with normal muscle tissues, we found 119 genes, including 8 expressed sequence tag (EST) genes with 3 folds differential gene expression among 17 000 genes in PM. A total of 64 genes and 47 genes were found up-regulated and down-regulated in PM, respectively. These genes were found to be associated with cell proliferation, signal transduction, cell cycle, gene transcription, and DNA damage and repair. RT-PCR and Western blot showed that the expression levels of NF-kappaB, TNF-alpha, and IL-1alpha genes were remarkably up-regulated in PM.
CONCLUSION: The development of PM might be regulated by multi-gene expression and NF-kappaB, TNF-alpha, and IL-1alpha genes might be candidate molecular markers of PM.