One health (Amsterdam, Netherlands) · 2026
Plasmid-borne mcr-4.3 in Acinetobacter nosocomialis recovered from imported beef cattle: Genomic characterization and one health implications.
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Xu Hao, Li Yaling, Hu Shaohua, Liu Ruishan, Wang Kun, Zheng Beiwen, Xiao Yonghong
Аннотация
BACKGROUND: The emergence of mobile colistin resistance determinants in clinically relevant Acinetobacter species poses a growing challenge to antimicrobial stewardship and One Health surveillance. Although mcr-4.3 has been reported in Acinetobacter, its occurrence in imported food animals and its broader phylogenomic context remain poorly defined.
METHODS: We investigated fecal samples collected from 150 beef cattle imported from Australia to China. Acinetobacter isolates recovered by selective culture were subjected to whole-genome sequencing, and the mcr-4.3-positive isolate was further resolved by hybrid assembly using short- and long-read sequencing. Comparative plasmid analysis and global phylogenomic analysis were performed using publicly available A. nosocomialis genomes.
RESULTS: Among 54 Acinetobacter isolates recovered from imported cattle, one A. nosocomialis isolate, NB148, carried mcr-4.3. Complete genome sequencing showed that mcr-4.3 was located on a 28,233-bp plasmid, designated pNB148_mcr-4.3, which harbored plasmid maintenance-associated toxin-antitoxin systems and shared substantial backbone similarity with previously reported mcr-4.3-bearing plasmids from Acinetobacter species. The mcr-4.3 region was positioned downstream of a putative recombinase and a Tn3-family transposase, suggesting a conserved genetic framework for this resistance determinant. Global phylogenomic analysis incorporating 574 publicly available A. nosocomialis genomes placed NB148 within a diverse international population characterized by mixing across animal, environmental, and human sources. Among the global collection, 11 genomes carried mcr-4.3, of which 10 belonged to ST279, indicating that this lineage may represent an important genomic background for the persistence and international distribution of mcr-4.3-positive A. nosocomialis.
CONCLUSIONS: This study documents plasmid-borne mcr-4.3 in A. nosocomialis recovered from imported beef cattle and places this finding within a broader international One Health context. Our data expand the known ecological range of mcr-4.3-positive A. nosocomialis and support strengthened genomic surveillance at animal-human-environment interfaces, particularly in importation and border inspection settings.
METHODS: We investigated fecal samples collected from 150 beef cattle imported from Australia to China. Acinetobacter isolates recovered by selective culture were subjected to whole-genome sequencing, and the mcr-4.3-positive isolate was further resolved by hybrid assembly using short- and long-read sequencing. Comparative plasmid analysis and global phylogenomic analysis were performed using publicly available A. nosocomialis genomes.
RESULTS: Among 54 Acinetobacter isolates recovered from imported cattle, one A. nosocomialis isolate, NB148, carried mcr-4.3. Complete genome sequencing showed that mcr-4.3 was located on a 28,233-bp plasmid, designated pNB148_mcr-4.3, which harbored plasmid maintenance-associated toxin-antitoxin systems and shared substantial backbone similarity with previously reported mcr-4.3-bearing plasmids from Acinetobacter species. The mcr-4.3 region was positioned downstream of a putative recombinase and a Tn3-family transposase, suggesting a conserved genetic framework for this resistance determinant. Global phylogenomic analysis incorporating 574 publicly available A. nosocomialis genomes placed NB148 within a diverse international population characterized by mixing across animal, environmental, and human sources. Among the global collection, 11 genomes carried mcr-4.3, of which 10 belonged to ST279, indicating that this lineage may represent an important genomic background for the persistence and international distribution of mcr-4.3-positive A. nosocomialis.
CONCLUSIONS: This study documents plasmid-borne mcr-4.3 in A. nosocomialis recovered from imported beef cattle and places this finding within a broader international One Health context. Our data expand the known ecological range of mcr-4.3-positive A. nosocomialis and support strengthened genomic surveillance at animal-human-environment interfaces, particularly in importation and border inspection settings.