The regulation characterization of novel transcription regulator JTY_2262 in Mycobacterium bovis BCG.

Mycobacterium tuberculosis (Mtb) can survive for a long time in vivo and evade host immune attacks, primarily through transcriptional regulation mediated by transcription regulators. JTY_2262 (homologous with Rv2250c) belongs to the Tetracycline Repressor (TetR) family of regulators, and its function is currently unclear. In this study, we combined JTY_2262 overexpressed transcriptomics and electrophoretic mobility shift assays (EMSA) to identify novel targets regulated by JTY_2262. The cofactors containing Cys, VC, VB1, VB3, VB6, Pb2+, Cu2+ and Li + inhibit the binding between JTY_2262 and the JTY_2045 (homologous with rv2031c) promoter. Overall, this study demonstrates that JTY_2262 is a versatile regulator. Under different environmental conditions, JTY_2262 senses concentration variations of specific cofactors. By modulating its DNA-binding affinity accordingly, JTY_2262 regulates targeted gene expression, enabling bacteria to adapt their metabolic machinery and physiological state to better adapt the changing environment.