Gene · 2026
Differential expression patterns of circulating NEAT1, HOTAIR, and miR-23b in rheumatoid arthritis: Implications for molecular diagnosis.
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Jumaa Shafaa Kadhim, Al-Khafaji Hiba Muneer Abdel Hassan
Аннотация
OBJECTIVE: This study aimed to investigate the expression of miR-23b and selected long non-coding RNAs (lncRNAs), including NEAT1 and HOTAIR, in patients with rheumatoid arthritis, and to examine the relationship between miR-23b and these lncRNAs in the regulation of inflammatory processes.
METHODS: A total of 100 peripheral blood samples were collected from patients with rheumatoid arthritis (n = 50) and healthy controls (n = 50). Total RNA was isolated from whole blood samples and reverse transcribed into complementary DNA (cDNA). The expression levels of miR-23b and selected lncRNAs were analyzed using quantitative real-time polymerase chain reaction. Relative gene expression was calculated, and statistical analyses were performed to compare expression levels between groups and to assess correlations among the studied molecular markers.
RESULTS: Significant downregulation of NEAT1 and HOTAIR was observed in rheumatoid arthritis (RA) patients compared to healthy control subjects (P < 0.001). In RA patients, miR-23b was significantly reduced compared to controls (P < 0.001). NEAT1 or HOTAIR expression did not correlate with disease duration, gender, or disease severity when used as a stratified analysis. In contrast, miR-23b expression was significantly correlated with disease severity, and the downregulation was greatest in severe RA patients (P < 0.05). Furthermore, the correlation analysis confirmed a significant negative association between miR-23b expression and inflammatory activity markers.
CONCLUSION: The present study demonstrates that NEAT1 and HOTAIR are significantly downregulated in patients with rheumatoid arthritis, highlighting their potential involvement in disease-associated inflammatory dysregulation. This suggests that both NEAT1 and HOTAIR may serve as promising non-invasive indicators for RA.
METHODS: A total of 100 peripheral blood samples were collected from patients with rheumatoid arthritis (n = 50) and healthy controls (n = 50). Total RNA was isolated from whole blood samples and reverse transcribed into complementary DNA (cDNA). The expression levels of miR-23b and selected lncRNAs were analyzed using quantitative real-time polymerase chain reaction. Relative gene expression was calculated, and statistical analyses were performed to compare expression levels between groups and to assess correlations among the studied molecular markers.
RESULTS: Significant downregulation of NEAT1 and HOTAIR was observed in rheumatoid arthritis (RA) patients compared to healthy control subjects (P < 0.001). In RA patients, miR-23b was significantly reduced compared to controls (P < 0.001). NEAT1 or HOTAIR expression did not correlate with disease duration, gender, or disease severity when used as a stratified analysis. In contrast, miR-23b expression was significantly correlated with disease severity, and the downregulation was greatest in severe RA patients (P < 0.05). Furthermore, the correlation analysis confirmed a significant negative association between miR-23b expression and inflammatory activity markers.
CONCLUSION: The present study demonstrates that NEAT1 and HOTAIR are significantly downregulated in patients with rheumatoid arthritis, highlighting their potential involvement in disease-associated inflammatory dysregulation. This suggests that both NEAT1 and HOTAIR may serve as promising non-invasive indicators for RA.