International immunopharmacology · 2025
Hypoxia-preconditioned cardiomyocyte-derived extracellular vesicles alleviate myocardial ischemic injury by reprogramming macrophage polarization via the Fgl2/NF-κB pathway.
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Zhang Zhibin, Shen Hechen, Zhang Shijie, Zhou Jianghui, Zhang Meng, Wang Yuchao, Zheng Yue, Chang Yun, Liang Xiaoyu, Hu Xiaomin, Gao Wenqing
Аннотация
BACKGROUND: Myocardial infarction (MI) elicits a robust inflammatory response that exacerbates cardiac injury and impairs functional recovery, making immunomodulation a key strategy for improving post-MI outcomes.
METHODS: We isolated extracellular vesicles (EVs) from normoxic and hypoxia/reoxygenation-treated cardiomyocytes, profiled their microRNA cargo, and examined their effects on LPS-stimulated RAW264.7 macrophages and a murine MI model using gain- and loss-of-function approaches targeting miR-199b-3p and fibrinogen-like protein 2 (Fgl2).
RESULTS: Hypoxia/reoxygenation-derived EVs were enriched in intraluminal miR-199b-3p and were efficiently taken up by macrophages; miR-199b-3p directly targeted Fgl2, suppressed TLR4/NF-κB activation, limited M1 polarization, restored phagocytic function, and reduced pro-inflammatory cytokine release. In MI mice, hypoxia/reoxygenation-EVs or a miR-199b-3p agomir decreased cardiac Fgl2 expression, inflammatory cytokine levels, infarct size, and apoptosis, and improved left ventricular function, whereas Fgl2 overexpression largely abrogated these benefits.
CONCLUSIONS: Cardiomyocyte-derived hypoxia/reoxygenation-EVs deliver miR-199b-3p to restrain Fgl2-dependent inflammatory signaling and post-infarction injury, highlighting the EV/miR-199b-3p/Fgl2 axis as a potential therapeutic target for improving outcomes after MI.
METHODS: We isolated extracellular vesicles (EVs) from normoxic and hypoxia/reoxygenation-treated cardiomyocytes, profiled their microRNA cargo, and examined their effects on LPS-stimulated RAW264.7 macrophages and a murine MI model using gain- and loss-of-function approaches targeting miR-199b-3p and fibrinogen-like protein 2 (Fgl2).
RESULTS: Hypoxia/reoxygenation-derived EVs were enriched in intraluminal miR-199b-3p and were efficiently taken up by macrophages; miR-199b-3p directly targeted Fgl2, suppressed TLR4/NF-κB activation, limited M1 polarization, restored phagocytic function, and reduced pro-inflammatory cytokine release. In MI mice, hypoxia/reoxygenation-EVs or a miR-199b-3p agomir decreased cardiac Fgl2 expression, inflammatory cytokine levels, infarct size, and apoptosis, and improved left ventricular function, whereas Fgl2 overexpression largely abrogated these benefits.
CONCLUSIONS: Cardiomyocyte-derived hypoxia/reoxygenation-EVs deliver miR-199b-3p to restrain Fgl2-dependent inflammatory signaling and post-infarction injury, highlighting the EV/miR-199b-3p/Fgl2 axis as a potential therapeutic target for improving outcomes after MI.