Abnormal numbers of cytokine producing cells in patients with polymyositis and dermatomyositis.

OBJECTIVE

To examine the constitutive pattern of cytokine production in patients with polymyositis (PM) and dermatomyositis (DM).

METHODS

PBMC secreting the type 1 cytokines IL-2 and IFN gamma and the type 2 cytokines IL-6 and IL-10 were monitored by ELIspot assay.

RESULTS

The frequency of IFN gamma-secreting PBMC was reduced 2-20 fold in patients with PM and DM when compared to normal controls (p < 0.01). This change correlated inversely with disease activity (p < 0.01). PM and DM were also characterized by increased numbers of cells secreting IL-10. Patients with DM had fewer IL-2 (p < 0.03) and more IL-6 (p < 0.006) secreting cells than normal controls or patients with other myopathies.

CONCLUSIONS

Abnormal patterns of cytokine production characterize patients with PM and DM.