Current research in microbial sciences · 2025
Reduced fitness and enhanced immunogenicity of M. tuberculosis ural family isolates limit global spread.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Minias Alina, Fiedorowicz Lidia, Szulc-Kiełbik Izabela, Kozińska Monika, Zabost Anna, Zygała-Pytlos Daria, Struś Katarzyna, Słomka Marcin, Lach Jakub, Strapagiel Dominik, Dziadek Jarosław, Augustynowicz-Kopeć Ewa
Аннотация
INTRODUCTION: Mycobacterium tuberculosis Lineage 4 (L4) includes the globally widespread Haarlem family and the geographically restricted Ural family, the latter primarily found north of the Black Sea. The biological basis for their differing global distributions remains unclear.
MATERIALS AND METHODS: We compared phenotypic characteristics of 12 genetically unlinked clinical isolates-six each from the Haarlem and Ural families-collected from individual patients in Poland. Growth kinetics, competitive fitness, intracellular replication in macrophages, hypoxia recovery, and host immune responses were evaluated using in vitro assays.
RESULTS: Ural isolates exhibited significantly slower growth and lower colony-forming units in competitive fitness assays compared to Haarlem isolates. In macrophage infection models, Ural strains showed impaired intracellular replication. Additionally, Ural isolates demonstrated a diminished capacity to resuscitate from hypoxia, indicating possible defects in dormancy recovery. Host immune assays revealed that Ural isolates induced higher levels of IL-12p40, suggesting stronger immune activation.
DISCUSSION: Our findings indicate that Ural isolates possess reduced growth fitness and increased immunogenicity compared to Haarlem isolates. These traits may limit their capacity for transmission and persistence in hosts, potentially explaining their restricted geographic distribution.
CONCLUSION: The study highlights the influence of M. tuberculosis genetic background on host-pathogen interactions and epidemiological success.
MATERIALS AND METHODS: We compared phenotypic characteristics of 12 genetically unlinked clinical isolates-six each from the Haarlem and Ural families-collected from individual patients in Poland. Growth kinetics, competitive fitness, intracellular replication in macrophages, hypoxia recovery, and host immune responses were evaluated using in vitro assays.
RESULTS: Ural isolates exhibited significantly slower growth and lower colony-forming units in competitive fitness assays compared to Haarlem isolates. In macrophage infection models, Ural strains showed impaired intracellular replication. Additionally, Ural isolates demonstrated a diminished capacity to resuscitate from hypoxia, indicating possible defects in dormancy recovery. Host immune assays revealed that Ural isolates induced higher levels of IL-12p40, suggesting stronger immune activation.
DISCUSSION: Our findings indicate that Ural isolates possess reduced growth fitness and increased immunogenicity compared to Haarlem isolates. These traits may limit their capacity for transmission and persistence in hosts, potentially explaining their restricted geographic distribution.
CONCLUSION: The study highlights the influence of M. tuberculosis genetic background on host-pathogen interactions and epidemiological success.