Journal of ethnopharmacology · 2026
Phytochemical profiling and system-level mechanistic insights into the anti-arthritic activity of Barleria cristata: Evidence from in-silico network pharmacology, molecular docking, in-vitro and in-vivo studies.
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Sahoo Subhasish, Pattanaik Sandesh Kumar, Barad Paresh Kumar, Mahapatra Jyoti Ranjan, Acharya Sandip Kumar, Pattanaik Sovan, Rath Diptirani
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Barleria cristata L. is traditionally used in Indian ethnomedicine to treat inflammation, joint pain, and arthritis. Although it is widely used in traditional medicine to manage inflammatory conditions, scientific validation of its anti-arthritic potential remains limited.
AIM OF THE STUDY: To investigate the potential anti-arthritic efficacy of the methanol extract of B. cristata (MEBC) using in-silico, in-vitro, and in-vivo experimental assays.
MATERIALS AND METHODS: Phytoconstituents of B. cristata were analysed using UPLC-QToF-MS. Then, a series of in-silico analyses were performed including network pharmacology and molecular docking to identify key targets and interactions. In-vitro studies were performed on LPS-stimulated RAW 264.7 macrophages to assess anti-inflammatory and antioxidant effects. Lastly, in-vivo CFA-induced arthritis model in rats was used to evaluate the therapeutic activity of MEBC.
RESULTS: From B. cristata, sixteen phytochemical constituents were tentatively identified. A network was constructed in which key active phytocompounds and the potential targets were analysed. GO and KEGG enrichment analyses were done simultaneously. KEGG Mapper indicated that the extract's anti-arthritic activity is primarily associated with modulation of the PI3K-AKT signalling pathway. Molecular docking further demonstrated that the selected compounds exhibited strong and favorable interactions with key proteins within this pathway. Furthermore, in-vitro and in-vivo investigations validated these findings, suggesting that MEBC attenuates inflammatory responses, improves arthritic parameters, and regulates PI3K-AKT signalling pathway.
CONCLUSION: MEBC exhibits potent anti-arthritic activity by modulating PI3K-AKT mediated inflammatory and oxidative pathways, supporting its traditional therapeutic use and potential as a complementary treatment for rheumatoid arthritis.
AIM OF THE STUDY: To investigate the potential anti-arthritic efficacy of the methanol extract of B. cristata (MEBC) using in-silico, in-vitro, and in-vivo experimental assays.
MATERIALS AND METHODS: Phytoconstituents of B. cristata were analysed using UPLC-QToF-MS. Then, a series of in-silico analyses were performed including network pharmacology and molecular docking to identify key targets and interactions. In-vitro studies were performed on LPS-stimulated RAW 264.7 macrophages to assess anti-inflammatory and antioxidant effects. Lastly, in-vivo CFA-induced arthritis model in rats was used to evaluate the therapeutic activity of MEBC.
RESULTS: From B. cristata, sixteen phytochemical constituents were tentatively identified. A network was constructed in which key active phytocompounds and the potential targets were analysed. GO and KEGG enrichment analyses were done simultaneously. KEGG Mapper indicated that the extract's anti-arthritic activity is primarily associated with modulation of the PI3K-AKT signalling pathway. Molecular docking further demonstrated that the selected compounds exhibited strong and favorable interactions with key proteins within this pathway. Furthermore, in-vitro and in-vivo investigations validated these findings, suggesting that MEBC attenuates inflammatory responses, improves arthritic parameters, and regulates PI3K-AKT signalling pathway.
CONCLUSION: MEBC exhibits potent anti-arthritic activity by modulating PI3K-AKT mediated inflammatory and oxidative pathways, supporting its traditional therapeutic use and potential as a complementary treatment for rheumatoid arthritis.