Enfermedades infecciosas y microbiologia clinica · 2002
[Influence of adjuvants on the ability of anti-Tbp antibodies to block transferrin binding, iron uptake and growth of Neisseria meningitis].
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ferreirós Carlos, Ferreiro Nuria, Criado M T
Аннотация
OBJECTIVE: To evaluate the effect of five adjuvants on the ability of specific anti-TbpA/B to block iron uptake in Neisseria meningitidis.
MATERIALS AND METHODS: Transferrin binding complexes (TbpA/B) purified from a TbpB isotype II Neisseria meningitidis strain were used to obtain sera with five different adjuvant formulations in mice in order to test the effect of the adjuvant on the ability of specific anti-TbpA/B antibodies to block transferrin binding, iron uptake and growth by meningococci.
RESULTS: Levels of anti-TbpA/B antibodies were relatively low (1:125 in most cases), the highest being obtained with the RAS adjuvant (1:3125). Despite the relatively low responses, all sera were able to significantly inhibit transferrin binding, iron uptake and growth in the homologous strain. Nevertheless, the effect on a strain with a TbpB isotype different from that of the immunizing strain was almost nil, a result in keeping with the described division of the meningococci into at least two TbpB groups (isotypes I and II).
CONCLUSIONS: In contrast to previous results for another important meningococcal protein, FbpA, the use of various adjuvants in the immunization of mice with TbpA/B complexes did not produce differences in the immune responses elicited, except in relation to antibody titers.
MATERIALS AND METHODS: Transferrin binding complexes (TbpA/B) purified from a TbpB isotype II Neisseria meningitidis strain were used to obtain sera with five different adjuvant formulations in mice in order to test the effect of the adjuvant on the ability of specific anti-TbpA/B antibodies to block transferrin binding, iron uptake and growth by meningococci.
RESULTS: Levels of anti-TbpA/B antibodies were relatively low (1:125 in most cases), the highest being obtained with the RAS adjuvant (1:3125). Despite the relatively low responses, all sera were able to significantly inhibit transferrin binding, iron uptake and growth in the homologous strain. Nevertheless, the effect on a strain with a TbpB isotype different from that of the immunizing strain was almost nil, a result in keeping with the described division of the meningococci into at least two TbpB groups (isotypes I and II).
CONCLUSIONS: In contrast to previous results for another important meningococcal protein, FbpA, the use of various adjuvants in the immunization of mice with TbpA/B complexes did not produce differences in the immune responses elicited, except in relation to antibody titers.