[Clinical application of bio-derived bone transplantation with tissue engineering technique: 7 year follow-up].

OBJECTIVE

To summarize the medium-term clinical result of bio-derived bone transplantation in orthopedics with tissue engineering technique.

METHODS

From December 2000 to June 2001, 10 cases of various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogenous osteoblasts from periosteum (1 x 10(6)/mL) with bio-derived bone scaffold following 3 to 7 days co-culture. Six men and 4 women were involved in this study, aged from 14 to 70 years with a median of 42 years. Among them, there were 2 cases of bone cyst, 1 case of non-union of old fracture, 6 cases of fresh comminuted fracture with bone defect, and 1 case of chronic suppurative osteomyelitis. The total weight of tissue engineered bone was 3-15 g in all the cases, averaged 7.3 g in each case.

RESULTS

The wound in all the case healed by first intention. For 7 year follow up, bone union was completed within 3.0 to 4.5 months in 9 cases, but loosening occurred and the graft was taken out 1 year after operation in 1 case. The X-ray films showed that 9 cases achieved union except one who received resection of the head of humerus. No obvious abnormalities were observed, and the function of affected limbs met daily life and work.

CONCLUSION

Bio-derived tissue engineered bone has good osteogenesis. No obvious rejection and other complications are observed in the clinical application.