Nature communications · 2024
Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yee Sook Wah, Ferrández-Peral Luis, Alentorn-Moron Pol, Fontsere Claudia, Ceylan Merve, Koleske Megan L, Handin Niklas, Artegoitia Virginia M, Lara Giovanni, Chien Huan-Chieh, Zhou Xujia, Dainat Jacques, Zalevsky Arthur, Sali Andrej, Brand Colin M, Wolfreys Finn D, Yang Jia, Gestwicki Jason E, Capra John A, Artursson Per, Newman John W, Marquès-Bonet Tomàs, Giacomini Kathleen M
Аннотация
SLC22A10 is an orphan transporter with unknown substrates and function. The goal of this study is to elucidate its substrate specificity and functional characteristics. In contrast to orthologs from great apes, human SLC22A10, tagged with green fluorescent protein, is not expressed on the plasma membrane. Cells expressing great ape SLC22A10 orthologs exhibit significant accumulation of estradiol-17β-glucuronide, unlike those expressing human SLC22A10. Sequence alignments reveal a proline at position 220 in humans, which is a leucine in great apes. Replacing proline with leucine in SLC22A10-P220L restores plasma membrane localization and uptake function. Neanderthal and Denisovan genomes show proline at position 220, akin to modern humans, indicating functional loss during hominin evolution. Human SLC22A10 is a unitary pseudogene due to a fixed missense mutation, P220, while in great apes, its orthologs transport sex steroid conjugates. Characterizing SLC22A10 across species sheds light on its biological role, influencing organism development and steroid homeostasis.