[Construction of DNA vaccine including a chimeric gene encoding flagellin and outer membrane protein antigen from Leptospira interrogons serovar lai].

OBJECTIVE

To construct a fusion expression vector for DNA vaccine, including a flagellin gene (flaB2) and an outer membrane protein gene (ompL1) to enhance the protective immunity of Leptospira interrogans serova Lai (Stain 017).

METHODS

The DNA fragments encoding flaB2 and ompL1 were amplified respectively by PCR and then fused. The obtained chimeric gene ompL1-flaB2 contained a synthetic linker of ten amino acids to sustain its space conformation.

RESULTS

Identified by restriction enzyme analysis, an insert fragment of 1.8 kb was demonstrated. FlaB2 and ompL1 respectively had the same sequence as reported by DNA sequencing analysis.

CONCLUSION

A fusion expression plasmid containing flaB2 and ompL1 was constructed.