Communications biology · 2025
A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bahr Jasmin, Poschmann Gereon, Jungmann Andreas, Busch Martin, Ding Zhaoping, Vogt Jens, Zalfen Ria, Steinhausen Julia, Euan Martínez Arlen Aurora, Wachtmeister Thorsten, Rickert Daniel, Lautwein Tobias, Alter Christina, Amrute Junedh M, Lavine Kory J, Köhrer Karl, Levkau Bodo, Most Patrick, Stühler Kai, Hesse Julia, Schrader Jürgen
Аннотация
Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.