Clinical, cosmetic and investigational dermatology · 2025
Cladribine Ameliorates Imiquimod Induced Murine Psoriasiform Dermatitis.
СтатьяНазвание и аннотация: Перевод в очереди
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Xue Jingwen, Shu Dan, Zhao Yi
Аннотация
BACKGROUND: Significant progress has been made in understanding the mechanisms of psoriasis, particularly the role of the interleukin (IL)-23/T-helper (Th) 17 axis, leading to novel, targeted therapies. However, many patients develop resistance to treatment over time. Thus, exploring new therapeutic strategies for severe refractory psoriasis remains crucial.
OBJECTIVE: To investigate the effect of cladribine on imiquimod induced psoriasiform dermatitis in mice.
METHODS: We established an imiquimod (IMQ)-induced psoriasiform dermatitis mouse model to investigate cladribine's effects on skin immune cells. Mice were allocated to five groups: Control, IMQ, High-dose cladribine (30mg/kg), Low-dose cladribine (20mg/kg), and Methotrexate. We assessed cumulative scores, skin pathology, immunohistochemistry, flow cytometry, and serum cytokines. We also studied cladribine's long-term efficacy by reapplying IMQ for a second round (7 days) after five half-lives of cladribine.
RESULTS: Cladribine significantly ameliorated symptoms and pathological features of IMQ-induced psoriasis in both high and low-dose groups, with efficacy comparable to methotrexate. Cladribine dose-dependently reduced Th17 and Th1 cell frequencies in psoriatic skin, along with associated cytokines. High-dose cladribine demonstrated sustained inhibition of IMQ-induced psoriasis.
CONCLUSION: These findings indicate that cladribine can ameliorate imiquimod-induced psoriasiform dermatitis in mice, exhibiting a dose-dependent and sustained therapeutic effect.
OBJECTIVE: To investigate the effect of cladribine on imiquimod induced psoriasiform dermatitis in mice.
METHODS: We established an imiquimod (IMQ)-induced psoriasiform dermatitis mouse model to investigate cladribine's effects on skin immune cells. Mice were allocated to five groups: Control, IMQ, High-dose cladribine (30mg/kg), Low-dose cladribine (20mg/kg), and Methotrexate. We assessed cumulative scores, skin pathology, immunohistochemistry, flow cytometry, and serum cytokines. We also studied cladribine's long-term efficacy by reapplying IMQ for a second round (7 days) after five half-lives of cladribine.
RESULTS: Cladribine significantly ameliorated symptoms and pathological features of IMQ-induced psoriasis in both high and low-dose groups, with efficacy comparable to methotrexate. Cladribine dose-dependently reduced Th17 and Th1 cell frequencies in psoriatic skin, along with associated cytokines. High-dose cladribine demonstrated sustained inhibition of IMQ-induced psoriasis.
CONCLUSION: These findings indicate that cladribine can ameliorate imiquimod-induced psoriasiform dermatitis in mice, exhibiting a dose-dependent and sustained therapeutic effect.