Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2024
Trace-elements driven up-regulation of secreted proteases expression in the human-pathogenic fungus Trichophyton rubrum.
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Blechert Oliver, Lan Shanyu, Xiong Shuzhen, Zou Yuning, Li Peicong, Hu Jiewei, Li Jiayuan, Zhan Ping
Аннотация
BACKGROUND: Trichophyton rubrum is a widespread human pathogenic fungus, colonizing keratinized tissue of outer body-parts. Thereby, the pathogen is relying on nutrients available from the host. The invasive mechanism of the pathogen is relaying on secreted proteases, which hydrolyze skin-proteins for subsequent up-take.
METHODS: In this study, we analyzed the gene expression of secreted proteases by RNAseq. In the results, we show the expression profile of 31 secreted protease genes under three conditions: keratin medium and keratin medium with trace-elements or with glucose.
RESULTS: By adding trace-elements to keratin medium, the expression of secreted proteases increased from 1.8 % to 3.3 %. Across all groups of secreted proteases, higher expression was observed. The genes SUB4, MEP1, MEP3, MEP5, MEP9, LAP1, LAP2 and MCPA were significantly stronger expressed, whereby MEP5 (∼6 fold) and SUB4 (∼5.8 fold) were strongest up-regulated.
DISCUSSION: We discuss the influence and significance of trace-elements on secreted proteases. Further, we speculate about the disturbed nutritional immunity in psoriatic and atopic skin as factor for increased risk of getting severe T. rubrum infections.
METHODS: In this study, we analyzed the gene expression of secreted proteases by RNAseq. In the results, we show the expression profile of 31 secreted protease genes under three conditions: keratin medium and keratin medium with trace-elements or with glucose.
RESULTS: By adding trace-elements to keratin medium, the expression of secreted proteases increased from 1.8 % to 3.3 %. Across all groups of secreted proteases, higher expression was observed. The genes SUB4, MEP1, MEP3, MEP5, MEP9, LAP1, LAP2 and MCPA were significantly stronger expressed, whereby MEP5 (∼6 fold) and SUB4 (∼5.8 fold) were strongest up-regulated.
DISCUSSION: We discuss the influence and significance of trace-elements on secreted proteases. Further, we speculate about the disturbed nutritional immunity in psoriatic and atopic skin as factor for increased risk of getting severe T. rubrum infections.