Proceedings of the National Academy of Sciences of the United States of America · 2019
Microbe-focused glycan array screening platform.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Geissner Andreas, Reinhardt Anika, Rademacher Christoph, Johannssen Timo, Monteiro João, Lepenies Bernd, Thépaut Michel, Fieschi Franck, Mrázková Jana, Wimmerova Michaela, Schuhmacher Frank, Götze Sebastian, Grünstein Dan, Guo Xiaoqiang, Hahm Heung Sik, Kandasamy Jeyakumar, Leonori Daniele, Martin Christopher E, Parameswarappa Sharavathi G, Pasari Sandip, Schlegel Mark K, Tanaka Hidenori, Xiao Guozhi, Yang You, Pereira Claney L, Anish Chakkumkal, Seeberger Peter H
Аннотация
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.